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Strain Detail Sheet

Strain Name:
ES Parental cell line E14Tg2a.4
Stock Number:
015890-UCD
Citation ID:
RRID:MMRRC_015890-UCD
Major Collection:
BayGenomics

Gene Information

Gene Details

Genetic Alterations
MMRRC Overview of the BayGenomics Collection
Genotype Determination
ES Cell Line
E14Tg2A (feeder free cell line derived from E14TG2a)
Images are of Feeder Independent Parental ES Cells E14Tg2a.4:
 

Strain Description

Strain of Origin
129P2/OlaHsd
Strain Development
Cells were derived from 129P2/OlaHsd.

Related Protocols

Strain Origin

Donor
BayGenomics, BayGenomics Consortium.
Primary Reference
Hooper M, Hardy K, Handyside A, Hunter S, Monk M. HPRT-deficient (Lesch-Nyhan) mouse embryos derived from germline colonization by cultured cells. Nature. 1987 Mar 19-25;326(6110):292-5. (Medline PMID: 3821905)

Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE. BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. Nucleic Acids Res. 2003 Jan 1;31(1):278-81. (Medline PMID: 12520002)

Colony and Husbandry Information

Special Considerations

ES Cell Sex and Genotype: These ES Cells are expected to be male (XY) and heterozygous/hemizygous for the mutation.

Health Status Report

Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment. The parental cell line E14Tg2a.4, from which these cells were derived, was tested for multiple mouse pathogens; please see the Health Status Report.

Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@ucdavis.edu.

Order Request Information

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
015890-UCD-CTRL BayGenomics High Passage Parental cell line ES Parental cell line E14Tg2a.4 (suitable for DNA extraction, control purposes; not for germline transmission) $148.67 / Non-Profit Vial Suitable for DNA extraction, control purposes but not for germline transmission.
015890-UCD-CELL BayGenomics Low Passage Parental cell line ES Parental cell line E14Tg2a.4 (suitable for electroporation and germline transmission) $740.70 / Non-Profit Vial Suitable for a single electroporation and germline transmission.
015890-UCD-ULTRA BayGenomics Ultra-low Passage Parental cell line ES Parental cell line E14Tg2a.4 (suitable for multiple electroporations and germline transmission) $2,059.70 / Non-Profit Vial Suitable for a multiple electroporation and germline transmission.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.