Loading...

Strain Detail Sheet

Strain Name:
B6.Cg-Anxa2tm2.1Kah/Mmjax
Stock Number:
071895-JAX
Citation ID:
RRID:MMRRC_071895-JAX
Other Names:
Anxa2LacZ/+

Strain Information

Gene Details

Anxa2tm2.1Kah
Name: annexin A2; targeted mutation 2.1, Katherine A Hajjar
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Knock-In
Anxa2
Name: annexin A2
Synonyms: lipocortin II, 36-kDa calelectrin, Cal1h, annexin II
Type: Gene
Species: Mouse
Chromosome: 9
Alteration at locus: Knock-In
NCBI: 12306
Homologene: 20857

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Anxa2LacZ/+ knock-in/knock-out (KI/KO) mice have a β-galactosidase gene (LacZ) replacing exons 3-4 of the Annexin A2 (Anxa2) gene, abolishing endogenous gene expression. These mice may be useful for bone marrow transplant studies used to characterize the role of Annexin A2 in recruitment of monocyte/macrophages to sites of injury.

Genotype Determination
ES Cell Line
CY2.4 derived from B6(Cg)-Tyrc-2J

Strain Description

Phenotype
The Anxa2 gene codes for Annexin A2, which is a calcium-dependent phospholipid-binding protein. Annexins are involved with cellular division, proliferation, migration, apoptosis, adhesion, exocytosis, endocytosis, and membrane trafficking. Anxa2 expression in vascular endothelial cells has been associated with activation of fibrinolysis as well as formation of new blood vessels. ANXA2 is also implicated in p53-mediated apoptosis and is upregulated in numerous cancers. These Anxa2LacZ/+ knock-in/knock-out mice express β-galactosidase under the direction of the Anxa2 promoter, while abolishing endogenous Anxa2 expression. Homozygous Anxa2LacZ/LacZ mice are viable and fertile with no overt phenotype.
MeSH Terms
  • Animals
  • Female
  • Humans
  • Male
  • Mice
  • Annexin A2/metabolism
  • Annexin A2/genetics
  • Cell Movement
  • Inflammation/metabolism
  • Inflammation/pathology
  • Macrophages/metabolism
  • Macrophages/immunology
  • Mice, Inbred C57BL
  • Retinal Pigment Epithelium/metabolism
  • Retinal Pigment Epithelium/pathology
  • Vitreoretinopathy, Proliferative/metabolism
  • Vitreoretinopathy, Proliferative/pathology
Strain Development
The Anxa2LacZ/+ targeting vector was designed to replace exons 3-4 of the annexin A2 (Anxa2) gene with a β-galactosidase gene (lacZ) and an Frt-flanked neomycin resistance (neo) cassette. The construct was electroporated into albino B6(Cg)-Tyrc-2J CY2.4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and the resulting chimeric mice were bred to C57BL/6 mice to confirm germline transmission. Anxa2LacZ/+ mice were then bred to a ROSA26::FLPe (JAX Stock No. 003946) knock in mouse line, on a 129S4/SvJaeSor background, to remove the Frt-flanked neomycin cassette. These mice, with neomycin cassette removed, were backcrossed to C57BL/6 for at least 10 generations by the donating laboratory (see SNP note below). Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish a live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes.

In 2024, a 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the males sent to The Jackson Laboratory Repository. One of the 43 markers throughout the genome, on Chromosome 8, was segregating with an unknown source.
Suggested Control Mice
C57BL/6J

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Cancer
  • Cardiovascular
  • Cell Biology
  • Diabetes
  • Immunology and Inflammation
  • Research Tools

Strain Origin

Donor
Katherine A. Hajjar, M.D., Cornell University Weill Cornell Medical College.
Primary Reference

Luo M, Almeida D, Dallacasagrande V, Hedhli N, Gupta M, D'Amico DJ, Kiss S, Hajjar KA. Annexin A2 promotes proliferative vitreoretinopathy in response to a macrophage inflammatory signal in mice. Nat Commun. 2024 Oct 9;15(1):8757. doi: 10.1038/s41467-024-52675-x. (Medline PMID: 39384746)

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcross
Generation
N/A
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
Greater than 15

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
071895-JAX-SPERM Cryo-preserved spermatozoa $459.00 / $459.00
Non-Profit / For-Profit		 Aliquot Approximate quantity3
071895-JAX-RESUS Litter recovered from cryo-archive $2,123.00 / $2,123.00
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.