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Strain Detail Sheet

Strain Name:
C57BL/6J-A1bgem1Flc/Mmnc
Stock Number:
071866-UNC
Citation ID:
RRID:MMRRC_071866-UNC
Other Names:
A1bg-flox

Strain Information

Gene Details

A1bgem1Flc
Name: alpha-1-B glycoprotein; endonuclease-mediated mutation 1, Frank Conlon
Synonyms: A1bgflox
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: CRISPR
A1bg
Name: alpha-1-B glycoprotein
Synonyms: C44
Type: Gene
Species: Mouse
Chromosome: 15
Alteration at locus: CRISPR
NCBI: 117586
VEGA: 15
HGNC: HGNC:5
Homologene: 11167

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Exons 4 to 7 were flanked by loxP sites via CRISPR/Cas9 technology.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    Not applicable

    Strain Description

    Phenotype
    Mice homozygous for the A1bgem1Flc allele appear normal in appearance and exhibit no obvious defects.

    Conditional phenotype: Offspring from crosses between MMRRC:71866 and STOCK Tg(Tnnt2-cre)5Blh/JiaoJ (JAX:24240) are viable and exhibit Mendelian inheritance ratios. A1bg expression is absent in cardiomyocyte lineages. In adult female A1BG-CKO mice, cardiac function is impaired, manifesting as incomplete closure of the outer left ventricular wall against the intraventricular septum during systole, accompanied by a thinned left ventricular wall. In contrast, adult male A1BG-CKO mice display normal cardiac function and physiology.

    MeSH Terms
    • Animals
    • Female
    • Genes, X-Linked/genetics
    • Gonads/growth & development
    • Gonads/metabolism
    • Male
    • Mice
    • Proteomics/methods
    • Sex Characteristics
    • Sex Chromosomes/metabolism
    Strain Development
    Cas9 guide RNAs targeting the mouse A1bg 3rd intron and downstream of the A1bg 7th exon were cloned into a T7 promoter vector followed by T7 in vitro transcription and spin column purification, with elution in microinjection buffer. C57BL/6J zygotes were microinjected with 400 nM Cas9 protein, 25 ng/ul each guide RNA and 20 ng/ul donor plasmid in microinjection buffer. Injected embryos were implanted in recipient pseudopregnant females. Resulting pups were screened by PCR for the presence of the loxP insertion events. A male founder was identified with homologous integration of the donor vector. The founder was mated to wild-type C57BL/6J females to establish a colony with the flox allele.
    Suggested Control Mice
    Wild-type littermate controls.

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Cardiovascular

    Strain Origin

    Donor
    Frank Conlon, Ph.D., University of North Carolina at Chapel Hill.
    Primary Reference

    Shi W, Sheng X, Dorr KM, Hutton JE, Emerson JI, Davies HA, Andrade TD, Wasson LK, Greco TM, Hashimoto Y, Federspiel JD, Robbe ZL, Chen X, Arnold AP, Cristea IM, Conlon FL. Cardiac proteomics reveals sex chromosome-dependent differences between males and females that arise prior to gonad formation. Dev Cell. 2021 Nov 8;56(21):3019-3034.e7. doi: 10.1016/j.devcel.2021.09.022. Epub 2021 Oct 15. (Medline PMID: 34655525)

    Colony and Husbandry Information

    Special Considerations

    N/A

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Sib-mating
    Generation
    N/A
    Overall Breeding Performance
    Excellent

    Reproductive Statistics

    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Hetero/Hemizygotes are fertile: Yes Yes
    Age Reproductive Decline: 7 to 9 months 10 to 12 months
    Average litter size
    7 to 9
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    7 to 9

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin.

    Conditions of Distribution

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    071866-UNC-RESUS Litter recovered from cryo-archive $3,088.00 / $5,126.00
    Non-Profit / For-Profit    		 Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.