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Strain Detail Sheet

Strain Name:
C57BL/6-Portm3Ding/Mmucd
Stock Number:
071816-UCD
Citation ID:
RRID:MMRRC_071816-UCD
Other Names:
CPR-low-reversible

Strain Information

Gene Details

Portm3Ding
Name: cytochrome p450 oxidoreductase; targeted mutation 3, Xinxin Ding
Synonyms: Cprr-CL
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Knock-In
Por
Name: cytochrome p450 oxidoreductase
Synonyms: NADH cytochrome P450 oxydoreductase, CYPOR, CPR, 4933424M13Rik
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Knock-In
NCBI: 18984
HGNC: HGNC:9208
Homologene: 725

Additional Resources
IMPC
Google
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Gene Ontology
BioGPS
Gene Cloud
IGTC
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KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
A floxed neo cassette was inserted into intron 15. The reduction in protein expression was confirmed by western blot analysis on liver, kidney, brain, lung, and olfactory mucosa extracts.
ES Cell Line
Bruce 4 derived from B6.Cg-Thy1a

Strain Description

Phenotype

Microsomal CPR protein levels were substantially decreased in all organs examined, in both males and females.

Adult male homozygotes had decreased body weight and decreased heart, lung, and kidney weights. Homozygous Cprlow females exhibit increased serum testosterone and progesterone, decreased copulatory activities, and infertility.

Conditional phenotype: Floxed neo gene

MeSH Terms
  • Animals
  • Body Weight
  • Cholesterol/blood
  • Embryo Loss/enzymology
  • Embryo Loss/genetics
  • Female
  • Fetal Development/genetics
  • Hepatocytes/enzymology
  • Infertility, Female/enzymology
  • Infertility, Female/genetics
  • Liver/embryology
  • Liver/enzymology
  • Male
  • Mice
  • Mice, Inbred Strains
  • Microsomes, Liver/enzymology
  • Models, Animal
  • NADPH-Ferrihemoprotein Reductase/biosynthesis
  • NADPH-Ferrihemoprotein Reductase/genetics
  • NADPH-Ferrihemoprotein Reductase/physiology
  • Organ Size
  • Organ Specificity
  • Progesterone/blood
  • Sex Characteristics
  • Testosterone/blood
  • Xenobiotics/metabolism
  • Xenobiotics/pharmacokinetics
Strain Development
The targeting construct for r-CL was a modified version of the targeting construct used for RRID:MMRRC_071815-UCD. The new construct contains the floxed neo marker inserted in Cpr intron 15, the homology arms that flank the neo cassette, and the tk gene needed for negative selection. The floxed neo marker would subsequently be inserted into intron 15 of the Cpr gene in the mouse genome via homologous recombination. Bruce 4 (B6.Cg-Thy1a) ES cells were used for electroporation. Following electroporation, cells were cultured and selected with G418. Individual G418 and ganciclovir-resistant embryonic stem cell clones were screened using PCR and Southern blot analysis. ES cells from positive clones were karyotyped and injected into albino B6(Cg)-Tyrc-2J/J blastocysts. The blastocysts were transferred into the uterus of a pseudopregnant B6CBAF1/J mouse to generate offspring. Adult chimeras were bred with WT B6 female mice to obtain germ-line-transmission F1 mice that were heterozygous for the mutant allele. The F2 homozygotes were obtained by intercrossing between the F1 heterozygotes. The structure of the modified Cpr gene in the r-CL mice was confirmed by PCR and Southern blot analysis.
Suggested Control Mice
B6

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Metabolism
  • Research Tools

Strain Origin

Donor
Xinxin Ding, Ph.D., University of Arizona.
Primary Reference

Wei Y, Zhou X, Fang C, Li L, Kluetzman K, Yang W, Zhang QY, Ding X. Generation of a mouse model with a reversible hypomorphic cytochrome P450 reductase gene: utility for tissue-specific rescue of the reductase expression, and insights from a resultant mouse model with global suppression of P450 reductase expression in extrahepatic tissues. J Pharmacol Exp Ther. 2010 Jul;334(1):69-77. doi: 10.1124/jpet.110.167411. Epub 2010 Apr 7. (Medline PMID: 20375200)

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Sib-mating
Generation
N/A
Overall Breeding Performance
Poor

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Sterile Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 7 to 9 months 7 to 9 months
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Robert Gallo.

Conditions of Distribution

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Robert Gallo

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
071816-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $882.81
Non-Profit / For-Profit		 Aliquot Approximate quantity3
071816-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.