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Strain Detail Sheet

Strain Name:
C57BL/6J-Naa10em1Lutzy/Mmjax
Stock Number:
071755-JAX
Citation ID:
RRID:MMRRC_071755-JAX
Other Names:
C57BL/6J-Naa10em1Lutzy/Mmjax

Strain Information

Gene Details

Naa10
Name: N(alpha)-acetyltransferase 10, NatA catalytic subunit
Synonyms: 2310039H09Rik, Te2, Ard1, Ard1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 56292
Homologene: 2608

Additional Resources
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KOMP Phenotyping
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Genetic Alterations
Naa10R83C knock-in (KI) mice harbor a CRISPR/Cas9-induced R83C missense variant and a silent R82R mutation in exon 5 of the N(alpha)-acetyltransferase 10, NatA catalyctic subunit (Naa10) gene. The Naa10 gene is part of a major N(alpha)-acetyltransferase complex responsible for alpha-acetylation of proteins and peptides. Mutations in this gene have been associated with developmental delay, attention-deficit/hyperactive disorder (ADHD) like behavior and cardiac abnormalities.
Genotype Determination
ES Cell Line
Not applicable

Strain Description

Phenotype
Heterozygous mice appear phenotypically normal and are fertile. Homozygous females and hemizygous males are sub-viable and born below expected Mendelian ratios (20.9% and 17.9%, respectively, from Het x Hemi matings). Homozygous females and hemizygous males are fertile but are occasionally non-productive in breeding. Hemizygous males are smaller in size with lower body weight than WT littermate males, but no differences are observed in heterozygous or homozygous females.
Strain Development
The Naa10R83C knock-in (KI) allele was generated using CRISPR/Cas9 endonuclease-mediated genome editing. A guide RNA (TCTGAGCCAGGCCAAGGCGC), and a single stranded oligo donor carrying the missense R83C (CGC>TGC, arginine to cysteine) variant and a silent R82R (CGG>AGA) mutation, were used for HDR-mediated genome editing. The R83C missense and R82R silent mutations are in exon 5 of the N(alpha)-acetyltransferase 10, NatA catalytic subunit (Naa10) gene on chromosome X. Mouse Naa10-201 transcript (ENSMUST00000033763.15) was used as reference for the exon number and guide sequences. Guide RNA, cas9, and the ss oligo donor were introduced to single cell C57BL/6J zygotes and transferred to pseudo pregnant females.
Suggested Control Mice
C57BL/6J or wildtype from colony

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Neurobiology

Strain Origin

Donor
Cat Lutz, Ph.D., The Jackson Laboratory.
Primary Reference
Not ready to publish

Colony and Husbandry Information

Special Considerations

Hom x Hemi and Het x WT

Health Status Report

For more information about this colony's health status contact csmmrrc@jax.org

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Sib-mating
Generation
2
Overall Breeding Performance
Good

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Reduced Reduced
Homozygotes are fertile: Reduced X-linked
Hetero/Hemizygotes are fertile: Yes Reduced
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Variable
Recommended wean age
4 Weeks
Average Pups Weaned
Variable

Order Request Information

Availability Level

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
071755-JAX-HOM-F
071755-JAX-HET-M
Homozygous Female
Heterozygous / Hemizygous Male
 $229.00 / $229.00
Non-Profit / For-Profit		 Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
071755-JAX-SPERM Cryo-preserved spermatozoa $459.00 / $459.00
Non-Profit / For-Profit		 Aliquot Approximate quantity3
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.