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Strain Detail Sheet

Strain Name:
CC061.129-Ace2tm1(ACE2)Bhk/Mmnc
Stock Number:
071655-UNC
Citation ID:
RRID:MMRRC_071655-UNC
Other Names:
CC061.Cg-ACE2^129S6-MmPrACE2/Unc

Strain Information

Gene Details

Ace2tm1(ACE2)Bhk
Name: angiotensin converting enzyme 2; targeted mutation 1, Beverly H Koller
Type: Allele
Species: Mus musculus
Chromosome: X
Alteration at locus: Targeted Mutation
Ace2
Name: angiotensin converting enzyme 2
Synonyms: 2010305L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Targeted Mutation
NCBI: 70008
Homologene: 41448

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
The targeting construct replaced the coding sequence through exon 18 with the human gene coding sequence.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    129Sv derived from 129S2/SvPas

    Strain Description

    Phenotype
    Mice expressing ACE2 in club cells under the control of the endogenous Ace2 promoter show a robust immune response after infection with SARS-CoV-2, leading to rapid clearance of the virus. This supports a model in which differential expression of ACE2 determines which cell types in the lung are infected, and this in turn modulates the host response and outcome of COVID-19.
    MeSH Terms
    • Animals
    • Humans
    • Mice
    • Angiotensin-Converting Enzyme 2/genetics
    • COVID-19/genetics
    • Mice, Transgenic
    • Receptors, Virus/genetics
    • SARS-CoV-2
    • Viral Tropism
    Strain Development
    Ace2 displacer constructs were assembled using a standard recombineering approach. The homologous arms were derived from the 129s7/AB2.2 bMQ BAC library clone and the ACE2 gene was derived from the human tile path BAC RP11-478H11. The resistance marker gene used for selection of embryonic stem cells consisted of a PGK-neo cassette flanked by mutant loxP sites. Excision of the marker gene with transient expression of Cre leaves a nonfunctional lox site in its place. Recombination for the generation of this line was carried out in the 129 ES cell line, Phnx43, derived from 129SvEv mice. ES cells carrying the correctly modified locus were used to generate chimeric mice, and bred to 129S6/SvEv/Tac mice to maintain the mutation on this genetic background. The offspring were used for examination of susceptibility to SARS-CoV-2.
    Suggested Control Mice
    heterozygous or wild type mice

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Models for Human Disease

    Strain Origin

    Donor
    Fernando Pardo Manuel De Villena, Ph.D., University of North Carolina-Chapel Hill.
    Primary Reference

    Snouwaert JN, Jania LA, Nguyen T, Martinez DR, Schäfer A, Catanzaro NJ, Gully KL, Baric RS, Heise M, Ferris MT, Anderson E, Pressey K, Dillard JA, Taft-Benz S, Baxter VK, Ting JP, Koller BH. Human ACE2 expression, a major tropism determinant for SARS-CoV-2, is regulated by upstream and intragenic elements. PLoS Pathog. 2023 Feb 22;19(2):e1011168. doi: 10.1371/journal.ppat.1011168. (Medline PMID: 36812267)

    Colony and Husbandry Information

    Special Considerations

    no

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    For more information about this colony's health status contact mmrrc_health@med.unc.edu

    Appearance

    Coat Color
    Agouti
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Other or uncertain
    Generation
    N=8
    Overall Breeding Performance
    Good

    Reproductive Statistics

    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: X-linked X-linked
    Homozygotes are fertile: Yes Yes
    Hetero/Hemizygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    4 to 6
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    4 to 6

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at mmrrc@med.unc.edu for more details.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    071655-UNC-SPERM Cryo-preserved spermatozoa $520.00 / $863.00
    Non-Profit / For-Profit    		 Aliquot Approximate quantity3
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.