Loading Mouse GIF
Loading...

Strain Name:
C57Bl/6J-Tg(CYP2A13,CYP2B6,CYP2F1)349Ding Del(7Cyp2b10-Cyp2b9)Fatso/FatsoMmnc
Stock Number:
071652-UNC
Citation ID:
RRID:MMRRC_071652-UNC
Other Names:
CYP2A13/2B6/2F1-Tg (on Cyp2b-null background)

Strain Information

Del(7Cyp2b10-Cyp2b9)1Fatso
Name: deletion, Chr 7, William Baldwin; deletion, Chr 7, William Baldwin
Synonyms: Cyp2b10em1(Del(7Cyp2b10-Cyp2b9))Fatso
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: CRISPR
Cyp2b9
Name: cytochrome P450, family 2, subfamily b, polypeptide 9
Synonyms: Cyp2b, phenobarbitol inducible, type a
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: CRISPR
NCBI: 13094
HGNC: HGNC:2615
Homologene: 74933
Cyp2b13
Name: cytochrome P450, family 2, subfamily b, polypeptide 13
Synonyms: phenobarbital inducible, type c
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: CRISPR
NCBI: 13089
HGNC: HGNC:2615
Homologene: 74933
Cyp2b10
Name: cytochrome P450, family 2, subfamily b, polypeptide 10
Synonyms: p16, Cyp2b, phenobarbitol inducible, type b, Cyp2b20
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: CRISPR
NCBI: 13088
HGNC: HGNC:2615
Homologene: 73894
BC026762
Name: cDNA sequence BC026762
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: CRISPR
Gm51474
Name: predicted gene, 51474
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: CRISPR
NCBI: 115486507
VEGA: 7
Cyp2b28-ps
Name: cytochrome P450, family 2, subfamily b, polypeptide 28, pseudogene
Type: Pseudogene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: CRISPR
Gm45227
Name: predicted gene 45227
Type: Pseudogene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: CRISPR
Gm45225
Name: predicted gene 45225
Type: Pseudogene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: CRISPR
Cyp2b27-ps
Name: cytochrome P450, family 2, subfamily b, polypeptide 27, pseudogene
Synonyms: EG667957
Type: Pseudogene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: CRISPR
Cyp2b26-ps
Name: cytochrome P450, family 2, subfamily b, polypeptide 26, pseudogene
Synonyms: EG623532
Type: Pseudogene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: CRISPR
Rnf170-ps
Name: ring finger protein 170, pseudogene
Synonyms: Gm8946
Type: Pseudogene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: CRISPR
Gm29920
Name: predicted gene, 29920
Type: Pseudogene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: CRISPR
Tg(CYP2A13,CYP2B6,CYP2F1)349Ding
Name: transgene insertion 349, Xinxin Ding
Type: Allele
Species: Multi-species
Chromosome: Unknown
Alteration at locus: Transgenic
CYP2A13/2B6/2F1
Name:
Type: Transgene
Species: Homo sapiens (human)
Chromosome:
Alteration at locus: Transgenic
Genetic Alterations
Human BAC clone CTD-2535H15 containing CYP2A13, CYP2B6, and CYP2F1 genes was linearized with NotI, and purified before microinjection into pronuclei of fertilized eggs. Transcripts of all three genes were confirmed immunologically.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    C57Bl/6J-693 derived from C57BL/6J
    Phenotype
    This mouse shows no phenotypic differences from wild-type mice when untreated. However, in the presence of a high-fat diet they may be slightly less obese while also demonstrating significantly more steatosis in the liver; similar phenotypes to FATP1-nulls or PPARg activation. They also metabolize some polyunsaturated fatty acids and most likely several xenobiotic chemicals differently as CYP2B6 is a key detoxification cytochrome P450.



    MeSH Terms
    • Humans
    • Male
    • Female
    • Mice
    • Animals
    • Oxylipins/metabolism
    • Cytochrome P-450 CYP2B6
    • Chromatography, Liquid
    • Mice, Inbred C57BL
    • Tandem Mass Spectrometry
    • Obesity/complications
    • Diet, High-Fat/adverse effects
    • Fatty Acids, Unsaturated/metabolism
    • Liver/metabolism
    • Non-alcoholic Fatty Liver Disease/metabolism
    • PPAR alpha/metabolism
    • Mice, Knockout
    • Fatty Acids/metabolism
    • Alkanesulfonic Acids/toxicity
    • Cytochrome P-450 CYP2B6/genetics
    • Cytochrome P-450 CYP2B6/metabolism
    • Diet, High-Fat
    • Fluorocarbons/toxicity
    • Gene Expression/drug effects
    • Liver/drug effects
    • Liver/pathology
    • Mice, Transgenic
    • Non-alcoholic Fatty Liver Disease/chemically induced
    • Non-alcoholic Fatty Liver Disease/pathology
    • Non-alcoholic Fatty Liver Disease/prevention & control
    • Triglycerides/metabolism
    Strain Development
    A human bacterial artificial chromosome containing CYP2A13, CYP2B6, and CYP2F1 genes was obtained from Invitrogen. The Purified BAC insert was microinjected into the pronuclei of fertilized eggs from the C57BL/6J strain. The eggs were transferred the same day or were cultured to the two-cell stage and then transferred into the oviducts of pseudopregnant B6CBAF1/J mice. Positive transgenic mice were identified through PCR. Transgenic mice were bred to Cyp2b-null mice resulting in transgenic mice that contain the human CYP2B6 gene on a Cyp2b9/10/13-null background. The null region includes 12 genes: Cyp2b10, Cyp2b28-ps, Gm45227, Gm45225, Cyp2b27-ps, BC026762, Gm51474, Cyp2b13, Rnf170-ps, Gm29920, Cyp2b26-ps, and Cyp2b9.
    Suggested Control Mice
    Controls can be the Cyp2b-null mice (MMRRC:050703-UNC. C57BL/6J-Cyp2b10em1(Del(7Cyp2b10-Cyp2b9))Fatso/Mmnc) or C57Bl/6 mice.
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Diabetes
    • Immunology and Inflammation
    • Internal/Organ
    • Metabolism
    • Models for Human Disease
    • Obesity
    Donor
    William Baldwin, Ph.D., Clemson University.
    Primary Reference

    Heintz MM, Eccles JA, Olack EM, Maner-Smith KM, Ortlund EA, Baldwin WS. Human CYP2B6 produces oxylipins from polyunsaturated fatty acids and reduces diet-induced obesity. PLoS One. 2022 Dec 15;17(12):e0277053. doi: 10.1371/journal.pone.0277053. (Medline PMID: 36520866)

    Hamilton MC, Heintz MM, Pfohl M, Marques E, Ford L, Slitt AL, Baldwin WS. Increased toxicity and retention of perflourooctane sulfonate (PFOS) in humanized CYP2B6-Transgenic mice compared to Cyp2b-null mice is relieved by a high-fat diet (HFD). Food Chem Toxicol. 2021 Jun;152:112175. doi: 10.1016/j.fct.2021.112175. Epub 2021 Apr 8. (Medline PMID: 33838175)

    Colony and Husbandry Information

    None. Sometimes we provide a higher fat diet to enhance breeding, but this is more common the Cyp2b-null controls to this humanized mouse.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Black
    Eye
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Generation
    backcrossed to WT B6 mice about 4-5 generations ago.
    Overall Breeding Performance
    Good
    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Hetero/Hemizygotes are fertile: Yes Yes
    Age Reproductive Decline: 7 to 9 months Greater than 12 months
    Average litter size
    4 to 6
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    4 to 6

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    071652-UNC-RESUS Litter recovered from cryo-archive $3,088.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.