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Strain Detail Sheet

Strain Name:
C57BL/6-Tg(Scgb1a1-Muc5ac/GFP)1Bouc/CehreMmnc
Stock Number:
071544-UNC
Citation ID:
RRID:MMRRC_071544-UNC
Other Names:
Muc5ac.GFP-TG

Strain Information

Gene Details

Tg(Scgb1a1-Muc5ac/GFP)1Bouc
Name: transgene insertion 1, Richard Boucher
Synonyms: Muc5ac-Tg
Type: Allele
Species: Multi-species
Chromosome: Unknown
Alteration at locus: Transgenic
GFP
Name: green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
Alteration at locus: Transgenic
Muc5ac
Name: mucin 5, subtypes A and C, tracheobronchial/gastric
Synonyms: MGM, 2210005L13Rik
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Transgenic
NCBI: 17833
HGNC: HGNC:7515
Homologene: 137237

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Scgb1a1
Name: secretoglobin, family 1A, member 1
Type: Gene
Species: Rattus norvegicus (norway rat)
Chromosome: 19
Alteration at locus: Transgenic
Genetic Alterations
A GFP coding sequence was inserted in-frame into the middle of the mouse Muc5ac coding sequence just 5 to the sequences encoding the variable numbers of tandem repeats (VNTR) domain. The expression of the fusion gene product was placed under the control of the rat Scgb1a1 promoter.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    Not specified

    Strain Description

    Phenotype

    Muc5ac-Tg mice suggests that mucin hypersecretion alone is not sufficient to trigger luminal mucus plugging or airway inflammation/goblet cell hyperplasia. Increased Muc5ac secretion appears to exhibit a protective role against influenza infection.

    Conditional phenotype: This strain does not have a conditional phenotype.

    MeSH Terms
    • Animals
    • Base Sequence
    • Blotting, Western
    • Bronchoalveolar Lavage Fluid/chemistry
    • Disease Models, Animal
    • Gene Expression
    • Green Fluorescent Proteins/genetics
    • Green Fluorescent Proteins/metabolism
    • HEK293 Cells
    • Host-Pathogen Interactions/immunology
    • Humans
    • Influenza A Virus, H1N1 Subtype/immunology
    • Influenza A Virus, H1N1 Subtype/physiology
    • Lung/immunology
    • Lung/metabolism
    • Lung/virology
    • Madin Darby Canine Kidney Cells
    • Mice
    • Mice, Inbred C57BL
    • Mice, Transgenic
    • Microscopy, Confocal
    • Microscopy, Electron
    • Molecular Sequence Data
    • Mucin 5AC/genetics
    • Mucin 5AC/immunology
    • Mucin 5AC/metabolism
    • Orthomyxoviridae Infections/immunology
    • Orthomyxoviridae Infections/metabolism
    • Orthomyxoviridae Infections/virology
    • Reverse Transcriptase Polymerase Chain Reaction
    Strain Development
    Transgenic mice with airway-specific overexpression of Muc5ac-GFP were generated by microinjection of fertilized C57BL/6 oocytes with the expression cassette pTG1-rCCSP-Muc5ac/GFP. Founder animals were bred with C57BL/6 mice, producing independent lines of Muc5ac-GFP transgenic mice. Transgene-positive animals were identified by PCR for GFP expression.
    Suggested Control Mice
    C57Bl6

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Immunology and Inflammation
    • Models for Human Disease

    Strain Origin

    Donor
    Camille Ehre, Ph.D., University of North Carolina at Chapel Hill.
    Primary Reference

    Ehre C, Worthington EN, Liesman RM, Grubb BR, Barbier D, O'Neal WK, Sallenave JM, Pickles RJ, Boucher RC. Overexpressing mouse model demonstrates the protective role of Muc5ac in the lungs. Proc Natl Acad Sci U S A. 2012 Oct 9;109(41):16528-33. doi: 10.1073/pnas.1206552109. Epub 2012 Sep 24. Erratum in: Proc Natl Acad Sci U S A. 2014 Apr 15;111(15):5753. (Medline PMID: 23012413)

    Colony and Husbandry Information

    Special Considerations

    N/A

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Random intra-strain mating
    Generation
    Unknown
    Overall Breeding Performance
    Excellent

    Reproductive Statistics

    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Undetermined Undetermined
    Homozygotes are fertile: Undetermined Undetermined
    Hetero/Hemizygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    7 to 9
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    7 to 9

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    - Products for this strain are Not Yet Available for Ordering
    - If you register interest in this strain, you will be notified when it becomes available for ordering.
    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.