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Conditional Npr2-7E cKI mice have a loxP-flanked EGFP reporter upstream of 7 glutamate substitutions (7E) in exons 8 and 9 of the Npr2 (natriuretic peptide receptor 2) gene. The 7E mutations prevent NPR2 dephosphorylation and inactivation. These floxed mice may be used as a tool for understanding signaling in mouse preovulatory follicles and bone growth regulation.
Of note, mice constitutively expressing the 7E mutations are available as B6;129-Npr2tm1.2Laj/Mmjax (MMRRC Stock No. 071312).
The Npr2-7E-cKI targeting vector was designed to insert a loxP site, a wild-type Npr2 minigene with 200 bp of intron 8 and cDNA from exons 8-22 (from BAC vector, RP24-306K11 and full length Npr2 cDNA), an IRES/EGFP reporter, an FRT-flanked PGK neomycin cassette and a loxP site into intron 7 and insert the 7E point mutations into exons 8 and 9 of the natriuretic peptide receptor 2 (Npr2) locus on chromosome 4. The 7E point mutations consist of 7 glutamate substitutions: S489E, S513E, T516E, S518E, S523E, S526E, T529E. The construct was electroporated into (129Sv x C57BL/6J)F1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. Chimeric males were crossed to B6.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/J females to remove the neomycin cassette. The flp allele was later bred out of the strain. The strain is maintained on a B6 and 129 mixed background. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes.
Shuhaibar LC, Egbert JR, Edmund AB, Uliasz TF, Dickey DM, Yee SP, Potter LR, Jaffe LA. Dephosphorylation of juxtamembrane serines and threonines of the NPR2 guanylyl cyclase is required for rapid resumption of oocyte meiosis in response to luteinizing hormone. Dev Biol. 2016 Jan 1;409(1):194-201. doi: 10.1016/j.ydbio.2015.10.025. Epub 2015 Oct 30. (Medline PMID: 26522847)
Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Technology Transfer Office.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Technology Transfer Office
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
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