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Strain Detail Sheet

Strain Name:
B6J.Cg-Dlk1tm2.1Stadt/Mmnc
Stock Number:
071305-UNC
Citation ID:
RRID:MMRRC_071305-UNC
Other Names:
Dlk1-Tomato/B6J

Strain Information

Gene Details

Dlk1tm2.1Stadt
Name: delta like non-canonical Notch ligand 1; targeted mutation 2.1, Matthias Stadtfeld
Synonyms: Dlk1Tom, Dlk1-Tomato
Type:
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Knock-In
Dlk1
Name: delta like non-canonical Notch ligand 1
Synonyms: pref-1, pG2, ZOG, SCP1, FA1, Peg9, DlkI
Type: Gene
Species: Mouse
Chromosome: 12
Alteration at locus: Knock-In
NCBI: 13386
HGNC: HGNC:2907
Homologene: 2846

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
tdTomato
Name:
Type: Gene
Species: Discosoma sp. (mushroom coral)
Chromosome:
Alteration at locus: Knock-In
Genetic Alterations
Insertion of IRES-tdTomato in 3'UTR of gene.
This tdTomato reporter allows visualization of the genomic imprinting from monoallelic regulators impact on the Dlk1 locus.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    v6.5

    Strain Description

    Phenotype

    In pluripotent stem cells (ESCs and iPSCs) derived from this mouse strain on the B6J background, loss of Dlk1 genomic imprinting occurs resulting in bi-allelic reporter expression. No such loss of imprinting is seen in animals on B6N and 129 backgrounds (see strain MMRRC:71307). Dlk1 expression leads to red fluorescence for visualization of Dlk1 in living pups.

    MeSH Terms
    • Animals
    • Calcium-Binding Proteins
    • Cell Culture Techniques
    • Cell Differentiation/drug effects
    • Cell Differentiation/genetics
    • Cells, Cultured
    • Embryo, Mammalian
    • Embryonic Development/genetics
    • Female
    • Genes, Reporter
    • Genetic Loci
    • Genomic Imprinting/drug effects
    • Genomic Instability/genetics
    • Intercellular Signaling Peptides and Proteins/genetics
    • Male
    • Mice
    • Mice, Inbred C57BL
    • Mice, Transgenic
    • Models, Genetic
    • Oxygen/pharmacology
    • Pluripotent Stem Cells/cytology
    • Pluripotent Stem Cells/drug effects
    • Pluripotent Stem Cells/metabolism
    • Ascorbic Acid/pharmacology
    • Calcium-Binding Proteins/genetics
    • Cell Line
    • Chromosomes, Mammalian/genetics
    • DNA Methylation/genetics
    • Embryonic Development/drug effects
    • Epigenesis, Genetic
    • Genomic Imprinting
    • Mouse Embryonic Stem Cells/metabolism
    • Quantitative Trait Loci/genetics
    Strain Development
    To generate targeting vectors for insertion of reporter genes into the 3’ UTR of Dlk1, a 6.7kb EcoRV fragment was isolated from BAC RP23-117C15 and cloned into pBluescript together with a floxed PGK-neoR cassette. In the resulting pBS-Dlk1, a SnaBI site was introduced at the site corresponding to position chr12:109,460,514 in mm10 using site-directed mutagenesis (Agilent QuikChange II). This site was used to insert the coding sequence of IRES-Venus or IRES-Tomato. V6.5 ESCs were electroporated with linearized (by SacII digestion) targeting vectors, followed by selection in G418-containing media. Correctly targeted clones were identified by Southern blotting of AseI-digested genomic DNA, using a 770bp HincII/AseI fragment probe isolated from pBS-Dlk1. Following confirmation, the PGK-neoR cassette was removed by transient transduction with Adeno-Cre. Resultant ESCs were injected into diploid blastocysts and transgenic chimeric mice bred with B6J animals to establish homozygous Dlk1 reporter mouse strains.

    Suggested Control Mice
    B6J

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Developmental Biology
    • Research Tools

    Strain Origin

    Donor
    Matthias Stadtfeld, Ph.D., Weill Cornell Medicine.
    Primary Reference

    Swanzey E, Stadtfeld M. A reporter model to visualize imprinting stability at the Dlk1 locus during mouse development and in pluripotent cells. Development. 2016 Nov 15;143(22):4161-4166. doi: 10.1242/dev.138255. Epub 2016 Oct 11. (Medline PMID: 27729406)

    Swanzey E, McNamara TF, Apostolou E, Tahiliani M, Stadtfeld M. A Susceptibility Locus on Chromosome 13 Profoundly Impacts the Stability of Genomic Imprinting in Mouse Pluripotent Stem Cells. Cell Rep. 2020 Mar 17;30(11):3597-3604.e3. doi: 10.1016/j.celrep.2020.02.073. (Medline PMID: 32187532)

    Colony and Husbandry Information

    Special Considerations

    no

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Backcross
    Generation
    9
    Overall Breeding Performance
    Good

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: N/A Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    Variable
    Recommended wean age
    4 Weeks
    Average Pups Weaned
    Variable

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Lisa Placanica.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Lisa Placanica

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    071305-UNC-RESUS Litter recovered from cryo-archive $3,088.00 / $5,126.00
    Non-Profit / For-Profit    		 Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.