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Strain Name:
B6J.Cg-Tg(Prop1-icre)432Sac/Mmucd
Stock Number:
070955-UCD
Citation ID:
RRID:MMRRC_070955-UCD
Other Names:
Tg(Prop1-cre)432Sac

Strain Information

icre
Name: codon-improved cre recombinase sequence
Type: Gene
Species: Enterobacteria phage P1 (bacteriophage P1)
Chromosome:
Prop1
Name: paired like homeodomain factor 1
Synonyms: prophet of Pit1, prophet of Pit-1, Prop-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 19127
HGNC: HGNC:9455
Homologene: 4558
Tg(Prop1-icre)432Sac
Name: transgene insertion 432, Sally A Camper
Synonyms: Prop1-cre
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: Unknown
Alteration at locus: Transgenic
Genetic Alterations
Transgenic line made with BAC (RP23-479M11) containing Prop1 gene that was engineered inserting Cre recombinase into exon 2, preserving the integrity of intron 1, as well as the spatial arrangement relative to the promoter.
Genotype Determination
ES Cell Line
Not applicable
Phenotype
This strain exhibits cre activity throughout the pituitary anterior lobe at 3 weeks.
MeSH Terms
  • Animals
  • Cell Lineage/genetics
  • Genetic Complementation Test
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • Humans
  • Immunohistochemistry
  • Mice, Inbred C57BL
  • Mice, Inbred Strains
  • Mice, Knockout
  • Mice, Transgenic
  • Microscopy, Fluorescence
  • Mutation
  • Pituitary Gland/cytology
  • Pituitary Gland/embryology
  • Pituitary Gland/metabolism
  • Pituitary Gland, Anterior/cytology
  • Pituitary Gland, Anterior/embryology
  • Pituitary Gland, Anterior/metabolism
  • Stem Cells/metabolism
Strain Development
A Prop1 containing BAC, RP23-479M11, which contains mouse genomic DNA similar to that shown to be sufficient for correcting the mutant phenotype. Using BAC recombineering, the cre coding sequence was inserted in-frame into exon 2, thus preserving the integrity of intron 1 completely, as well as the spatial arrangement of exon 2 relative to the promoter. The purified Prop1-cre modified BAC was injected into the pronuclei of fertilized eggs from a cross between C57BL/6J and B6D2F1 mice, and the fertilized eggs were transferred into pseudopregnant foster mothers. Three transgenic lines were generated and tested for cre activity: MMRRC:70955 Tg(Prop1-cre)432Sac and two others. All 3 lines exhibit cre activity throughout the pituitary anterior lobe at 3 weeks.
Suggested Control Mice
littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell Biology
  • Developmental Biology
  • Endocrine Deficiency
  • Internal/Organ
  • Reproduction
  • Research Tools
Donor
Sally A. Camper, Ph.D., University of Michigan.
Primary Reference

Davis SW, Keisler JL, Pérez-Millán MI, Schade V, Camper SA. All Hormone-Producing Cell Types of the Pituitary Intermediate and Anterior Lobes Derive From Prop1-Expressing Progenitors. Endocrinology. 2016 Apr;157(4):1385-96. doi: 10.1210/en.2015-1862. Epub 2016 Jan 26. (Medline PMID: 26812162)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross
Generation
unknown
Overall Breeding Performance
Excellent
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: 7 to 9 months 10 to 12 months
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
070955-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.