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Strain Name:
C57BL/6J-Fcgrb2em1(FCGRB2)SynblFcgr4em1(FCGR3A)Synbl/Mmnc
Stock Number:
069776-UNC
Citation ID:
RRID:MMRRC_069776-UNC
Other Names:
Humanized Mouse-hFcgr2b-hFcgr3a

Strain Information

Fcgr2bem1(FCGR2B)Synbl
Name: Fc receptor, IgG, low affinity IIb; endonuclease-mediated mutation 1, Synbal
Synonyms: Humanized Mouse-hFcgr2b
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Endonuclease-mediated
Fcgr4em1(FCGR3A)Synbl
Name: Fc receptor, IgG, low affinity IV; endonuclease-mediated mutation 1, Synbal
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Endonuclease-mediated
Fcgr4
Name: Fc receptor, IgG, low affinity IV
Synonyms: CD16-2, 4833442P21Rik, FcgammaRIV, Fcgr3a
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Endonuclease-mediated
NCBI: 246256
Homologene: 477
Fcgr2b
Name: Fc receptor, IgG, low affinity IIb
Synonyms: CD32, FcgRII, Fc[g]RII, Fc gamma RIIB, FcgammaRIIB, Fcgr2, Fcr-2, Ly-m20, Ly-17, LyM-1, Fcr-3, Fcgr2a, F630109E10Rik
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Endonuclease-mediated
NCBI: 14130
Homologene: 2974
Genetic Alterations
Using a CRISPR/Cas9 approach, an AGC (active genetic cassettes - a marker of interest plus a guide-RNA (gRNA) cassette configured to promote expression) was used to remove the endogenous mouse gene coding regions (Fcgr2b and Fcgr4) and replace it with a Kozak consensus translational initiation sequence, functional human FCGR2B cDNA min-gene cassette (HsFCGR2B), and FCGR3A coding regions under the control of the endogenous mouse promoters.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    Not applicable
    Phenotype
    Human FCGR3A is expressed under control of the endogenous mouse Fcgr4 promoter. In addition, human FCGR2B is expressed under control of the endogenous mouse Fcgr2b promoter.
    Strain Development
    To generate a murine Fc fragment receptor model with a human gene knock-in, a CRISPR/Cas9-assisted gene targeting strategy was used. This involved creating a knockout (KO) of the mouse Fc fragment receptor gene while concurrently inserting a human Fc fragment receptor mini-gene under the control of the mouse promoter. The following sequences were introduced at the start codon in mouse exon 2: A Kozak sequence and human cDNA cassette inserted at the cleavage site, Sv40 intron, a 214 bp native FCGRT 3' untranslated region, a 152 bp native human sequence, 3 of the mapped 3UTR, and a mouse U6 promoter-driven guide RNA scaffold, a splice-trap element with splice acceptor and SV40 polyadenylation sequence followed by 3-frame ATG short open reading frames. This active genetics cassette is designed to trap and truncate any mouse Fcgrt isoforms that may splice past the cassette. Additionally, a Flp Recombinase Target (FRT) site is inserted to facilitate Flp-mediated collapse of targeted tandem integration events. All experiments were conducted in C57BL/6J mice.
    Suggested Control Mice
    C57BL/6J
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cancer
    • Dermatology
    • Hematology
    • Immunology and Inflammation
    • Models for Human Disease
    • Reproduction
    • Research Tools
    • Virology
    Donor
    Kurt Jarnagin, Ph.D., Synbal, Inc..
    Primary Reference

    BioRxiv Doi:10.1101/2022.08.30.505951 ROBUST AND EFFICIENT ACTIVE GENETICS GENE CONVERSION IN THE RAT AND MOUSEChenyen Lai, Oscar Alvarez, Kristen Read, Don van Fossan, Christopher M Conner, Shannon Xaing-Ru Xu, Dale O. Cowley, Valentino Gantz, David R. Webb, Kurt Jarnagin

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    White
    Eye
    Pink
    MMRRC Breeding System
    Sib-mating
    Generation
    N/A
    Overall Breeding Performance
    Good
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    7 to 9
    Recommended wean age
    4 Weeks
    Average Pups Weaned
    Undetermined

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    069776-UNC-EMBRYO Cryo-preserved embryos $1,488.00 / $2,470.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity2 : 20-40 embryos / aliquot
    069776-UNC-SPERM Cryo-preserved spermatozoa $520.00 / $863.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity3
    069776-UNC-RESUS Litter recovered from cryo-archive $3,088.00 / $5,126.00
    Non-Profit / For-Profit
    Litter Recovered litter4; additional fees for any special requests.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.