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Strain Detail Sheet

Strain Name:
B6J.Cg-Ccn1tm2Lfl/Mmmh
Stock Number:
069625-MU
Citation ID:
RRID:MMRRC_069625-MU
Other Names:
Ccn1-DM/DM

Strain Information

Gene Details

Ccn1tm2Lfl
Name: cellular communication network factor 1; targeted mutation 2, Lester F Lau
Synonyms: CCN1dm
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Knock-In
Ccn1
Name: cellular communication network factor 1
Synonyms: CCN1, Cyr61
Type: Gene
Species: Mouse
Chromosome: 3
Alteration at locus: Knock-In
NCBI: 16007
HGNC: HGNC:2654
Homologene: 1194

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Exon 1 and half of exon 2 of the Ccn1 gene were replaced by a DNA cassette containing the Cyr61 promoter, the dm (apoptosis defective mutant allele) open reading frame (containing the signal sequence, IGFBP, VWC, variable, TSP, and CT domains with H1 and H2 sites of Cyr61), and a neo cassette. The dm open reading frame is defective in apoptotic synergism with TNFalpha. Seven lysines and one arginine in the H1 and H2 sites of CCN1 were changed to alanine, abolishing CCN1 binding to integrin α6β1 and heparan sulfate proteoglycans (PMID:15322081). Genetic alteration information at the nucleic acid level is unavailable.
HVGS Nomenclature
ES Cell Line
J1 derived from 129S4/SvJae

Strain Description

Phenotype
This knock-in replaces the Ccn1 gene with a mutant allele with mutations that abrogate CCN1 binding to integrin α6β1. Ccn1-DM/DM mice are viable and fertile. They do not have obvious phenotypes in homeostasis, but upon injury, they exhibit a broad range of defects in injury repair and tissue regeneration.
MeSH Terms
  • Animals
  • Apoptosis/drug effects
  • Caspase 3/genetics
  • Caspase 3/metabolism
  • Cells, Cultured
  • Cysteine-Rich Protein 61
  • Fibroblasts/cytology
  • Fibroblasts/drug effects
  • Fibroblasts/metabolism
  • Humans
  • Immediate-Early Proteins/genetics
  • Immediate-Early Proteins/metabolism
  • Infant, Newborn
  • Integrins/metabolism
  • JNK Mitogen-Activated Protein Kinases/genetics
  • JNK Mitogen-Activated Protein Kinases/metabolism
  • Mice
  • Mice, Knockout
  • Models, Biological
  • Mutation
  • NADPH Oxidases/genetics
  • NADPH Oxidases/metabolism
  • NF-kappa B/metabolism
  • RNA, Small Interfering/genetics
  • Reactive Oxygen Species/metabolism
  • Signal Transduction/drug effects
  • Tumor Necrosis Factor-alpha/metabolism
  • Tumor Necrosis Factor-alpha/pharmacology
  • Blotting, Western
  • Cell Line
  • Cell Proliferation/drug effects
  • Cellular Senescence/drug effects
  • Collagen/metabolism
  • Cysteine-Rich Protein 61/pharmacology
  • Fibroblasts
  • Mice, Inbred C57BL
  • Microscopy, Fluorescence
  • RNA Interference
  • Reverse Transcriptase Polymerase Chain Reaction
  • Wound Healing/drug effects
  • Carotid Artery Diseases/diagnosis
  • Carotid Artery Diseases/metabolism
  • Carotid Artery Diseases/pathology
  • Carotid Artery Diseases/physiopathology
  • Carotid Artery, Common/metabolism
  • Carotid Artery, Common/pathology
  • Carotid Artery, Common/physiopathology
  • Cysteine-Rich Protein 61/genetics
  • Cysteine-Rich Protein 61/metabolism
  • Disease Models, Animal
  • Disease Progression
  • Endothelial Cells/metabolism
  • Endothelial Cells/pathology
  • Human Umbilical Vein Endothelial Cells/metabolism
  • Integrin alpha6beta1/metabolism
  • Mechanotransduction, Cellular
  • Mice, Knockout, ApoE
  • Phenotype
  • Plaque, Atherosclerotic
  • Regional Blood Flow
  • Stress, Mechanical
  • Colitis/metabolism
  • Colitis/pathology
  • Enzyme-Linked Immunosorbent Assay
  • Fluorescent Antibody Technique
  • In Situ Nick-End Labeling
  • Interleukin-6/metabolism
  • Intestinal Mucosa/injuries
  • Intestinal Mucosa/metabolism
  • Intestinal Mucosa/pathology
  • Wound Healing/physiology
  • Alleles
  • Embryonic Stem Cells
  • Gene Order
  • Gene Targeting
  • Genetic Loci
  • Mice, Transgenic
  • Plasmids/genetics
Strain Development
This strain was created in the (129X1/SvJ x C57BL/6J) mixed background and has been backcrossed to the C57BL/6J background >11 times.
Suggested Control Mice
C57BL/6J

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Apoptosis
  • Cancer
  • Cell Biology
  • Dermatology
  • Immunology and Inflammation
  • Models for Human Disease

Strain Origin

Donor
Lester Lau, Ph.D., University of Illinois at Chicago.
Primary Reference

Monzon RI, Kim KH, Lau LF. Construction and Analysis of an Allelic Series of Ccn1 Knockin Mice. Methods Mol Biol. 2017;1489:361-376. doi: 10.1007/978-1-4939-6430-7_30. (Medline PMID: 27734389)

Additional References

Leu SJ, Chen N, Chen CC, Todorovic V, Bai T, Juric V, Liu Y, Yan G, Lam SC, Lau LF. Targeted mutagenesis of the angiogenic protein CCN1 (CYR61). Selective inactivation of integrin alpha6beta1-heparan sulfate proteoglycan coreceptor-mediated cellular functions. J Biol Chem. 2004 Oct 15;279(42):44177-87. doi: 10.1074/jbc.M407850200. Epub 2004 Aug 17. (Medline PMID: 15322081)

Chen CC, Young JL, Monzon RI, Chen N, Todorović V, Lau LF. Cytotoxicity of TNFalpha is regulated by integrin-mediated matrix signaling. EMBO J. 2007 Mar 7;26(5):1257-67. doi: 10.1038/sj.emboj.7601596. Epub 2007 Feb 22. (Medline PMID: 17318182)

Jun JI, Lau LF. The matricellular protein CCN1 induces fibroblast senescence and restricts fibrosis in cutaneous wound healing. Nat Cell Biol. 2010 Jul;12(7):676-85. doi: 10.1038/ncb2070. Epub 2010 Jun 6. Erratum in: Nat Cell Biol. 2010 Dec;12(12):1249. (Medline PMID: 20526329)

Hsu PL, Chen JS, Wang CY, Wu HL, Mo FE. Shear-Induced CCN1 Promotes Atheroprone Endothelial Phenotypes and Atherosclerosis. Circulation. 2019 Jun 18;139(25):2877-2891. doi: 10.1161/CIRCULATIONAHA.118.033895. Epub 2019 Mar 28. (Medline PMID: 30917686)

Choi JS, Kim KH, Lau LF. The matricellular protein CCN1 promotes mucosal healing in murine colitis through IL-6. Mucosal Immunol. 2015 Nov;8(6):1285-96. doi: 10.1038/mi.2015.19. Epub 2015 Mar 25. (Medline PMID: 25807183)

Colony and Husbandry Information

Special Considerations

No special diet or housing is required.

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcross
Generation
N11+ (C57BL/6J), F20+
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Greater than 12 months Greater than 12 months
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
069625-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
069625-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.