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Strain Detail Sheet

Strain Name:
STOCK Tg(FEZF2-cre/ERT2/EGFP)Pred3Jlr/Mmucd
Stock Number:
068100-UCD
Citation ID:
RRID:MMRRC_068100-UCD
Other Names:
Fezf2En1-CreERT2-GFP

Strain Information

Gene Details

Tg(FZEF2-cre/ERT2,-GFP)Pred3Jlr
Name: transgene insertion Pred3, John Rubenstein
Type: Allele
Species: Multi-species
Chromosome: unknown
GFP
Name: green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
cre/ERT2
Name: cre tamoxifen-dependent recombinase
Type: Gene
Species: Multi-species
Chromosome:
FEZF2
Name: FEZ family zinc finger 2
Type: Gene
Species: Homo sapiens (human)
Chromosome: 3
Genetic Alterations

Randomly inserted transgenic human FEZF2 enhancer sequence drives CreER and GFP expression, no genes are altered

Strain Description

Phenotype
This line carries an allele that encodes a novel FEZF2 enhancer driving the expression of CreER-T2 and GFP.
Strain Development
This transgenic line was created by pronuclear injection into fertilized CD-1 eggs. Three subsequent crosses to CD-1 and one sib-mating.
Suggested Control Mice
CD-1

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Developmental Biology
  • Models for Human Disease
  • Neurobiology

Strain Origin

Donor
John Rubenstein, M.D., University of California, San Francisco.
Primary Reference

Markenscoff-Papadimitriou E, Whalen S, Przytycki P, Thomas R, Binyameen F, Nowakowski TJ, Kriegstein AR, Sanders SJ, State MW, Pollard KS, Rubenstein JL. A Chromatin Accessibility Atlas of the Developing Human Telencephalon. Cell. 2020 Aug 6;182(3):754-769.e18. doi: 10.1016/j.cell.2020.06.002. Epub 2020 Jun 30. (Medline PMCID: 7415678)

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@ucdavis.edu

Appearance

Coat Color
White
Eye
Pink

Breeding

MMRRC Breeding System
Outcross to non-inbred (hybrid or outbred) mate
Generation
G3, F3
Overall Breeding Performance
Excellent

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
10 to 14
Recommended wean age
3 Weeks
Average Pups Weaned
10 to 14

Order Request Information

Availability Level

The availability level for this product has not been determined.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Tanya Eliason.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Tanya Eliason

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.