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Strain Detail Sheet

Strain Name:
B6J.C3-Sst1C3HeB/FejKrmn/Mmnc
Stock Number:
043908-UNC
Citation ID:
RRID:MMRRC_043908-UNC
Other Names:
B6.C3H-sst1

Strain Information

Gene Details

Sp110
Name: Sp110 nuclear body protein
Synonyms: 52kDa, Ipr1, 5830484A20Rik, 5031415C07Rik, Ifi75
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 109032
HGNC: HGNC:5401
Homologene: 82192

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Sst1C3HeB/FeJ
Name: susceptibility to tuberculosis 1; C3HeB/FeJ
Type: QTL
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Spontaneous Mutation
Sst1
Name: susceptibility to tuberculosis 1
Type: QTL
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Spontaneous Mutation
Genetic Alterations
The sst1 locus encompasses the HSR repeat encoding the repeat-containing Sp110 open reading frame (ORF). The full-length Sp110 ORF and corresponding protein Sp110/Lpr1 are completely lacking only in the C3HeB/FeJ substrain of C3H (PMID:15815631). The sst1 locus has been transferred from C3HeB-FeJ to C57BL/6J using 12 backcrosses. The B6.C3H-sst1 mice do not express the Sp110/Ipr1 ORF and protein.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.

    • Strain Description

    Phenotype

    Homozygous: Mice develop necrotic lung lesions after infection with intracellular bacteria. Macrophages display dysregulated response to TNF characterized by escalating stress and type-I interferon pathway activation.

    Hetero/Hemizygous: Not characterized. The sst1 susceptibility allele is recessive


    MeSH Terms
    • Animals
    • Chronic Disease
    • Disease Models, Animal
    • Genetic Predisposition to Disease
    • Granuloma
    • Humans
    • Immunohistochemistry
    • Inflammation/genetics
    • Inflammation/immunology
    • Macrophages/immunology
    • Mice
    • Mice, Congenic
    • Mice, Inbred C57BL
    • Recurrence
    • Reverse Transcriptase Polymerase Chain Reaction
    • Tuberculosis, Pulmonary/genetics
    • Tuberculosis, Pulmonary/immunology
    • Tuberculosis, Pulmonary/pathology
    • Antigens, Bacterial/biosynthesis
    • Antigens, Bacterial/genetics
    • Antigens, Bacterial/metabolism
    • Gene Expression Regulation, Bacterial/immunology
    • Gene Targeting/methods
    • Genome-Wide Association Study/methods
    • Mice, Inbred C3H
    • Mycobacterium tuberculosis/genetics
    • Mycobacterium tuberculosis/immunology
    • Reproducibility of Results
    • T-Lymphocyte Subsets/immunology
    • T-Lymphocyte Subsets/metabolism
    • T-Lymphocyte Subsets/microbiology
    • Tuberculosis Vaccines/genetics
    • Tuberculosis Vaccines/immunology
    • Tuberculosis Vaccines/therapeutic use
    • Tuberculosis, Pulmonary/microbiology
    • Antitubercular Agents/therapeutic use
    • Carcinoma, Squamous Cell/drug therapy
    • Carcinoma, Squamous Cell/etiology
    • Carcinoma, Squamous Cell/genetics
    • Cell Transformation, Neoplastic/genetics
    • Epidermal Growth Factor/genetics
    • Epiregulin
    • Female
    • Gene Expression
    • Genetic Predisposition to Disease/genetics
    • Host-Pathogen Interactions
    • Isoniazid/therapeutic use
    • Lung/metabolism
    • Lung/microbiology
    • Lung/pathology
    • Lung Neoplasms/drug therapy
    • Lung Neoplasms/etiology
    • Lung Neoplasms/genetics
    • Macrophages/metabolism
    • Macrophages/microbiology
    • Male
    • Mice, Inbred Strains
    • Mycobacterium tuberculosis/drug effects
    • Mycobacterium tuberculosis/physiology
    • Time Factors
    • Tuberculosis, Pulmonary/complications
    • Tuberculosis, Pulmonary/drug therapy
    • Caspase 3/genetics
    • Caspase 3/immunology
    • Chlamydophila Infections/genetics
    • Chlamydophila Infections/immunology
    • Chlamydophila Infections/pathology
    • Chlamydophila pneumoniae/genetics
    • Chlamydophila pneumoniae/immunology
    • Chlamydophila pneumoniae/ultrastructure
    • Genetic Loci/immunology
    • Immune Evasion/genetics
    • Immunity, Innate/physiology
    • Interferon-beta/genetics
    • Interferon-beta/immunology
    • Interleukin-10/genetics
    • Interleukin-10/immunology
    • Macrophages, Alveolar/immunology
    • Macrophages, Alveolar/ultrastructure
    • Pneumonia, Bacterial/genetics
    • Pneumonia, Bacterial/immunology
    • Pneumonia, Bacterial/pathology
    Strain Development
    (C3HeB/FeJxC57BL/6J)F1 obtained from JAX were backcrossed on C57BL/6J using speed congenics protocol for the first 6 generations and for a total of 12 backcross generations and a homozygous mouse strain has been established and maintained in the Kramnik laboratory.
    Suggested Control Mice
    Wild-type littermates

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Apoptosis
    • Cancer
    • Immunology and Inflammation
    • Models for Human Disease

    Strain Origin

    Donor
    Igor Kramnik, Ph.D., Boston University.
    Primary Reference

    Pichugin AV, Yan BS, Sloutsky A, Kobzik L, Kramnik I. Dominant role of thesst1 locus in pathogenesis of necrotizing lung granulomas during chronictuberculosis infection and reactivation in genetically resistant hosts. Am JPathol. 2009 Jun;174(6):2190-201. doi: 10.2353/ajpath.2009.081075. Epub 2009 May 14. (Medline PMID: 19443700)

    Commandeur S, van Meijgaarden KE, Prins C, Pichugin AV, Dijkman K, van denEeden SJ, Friggen AH, Franken KL, Dolganov G, Kramnik I, Schoolnik GK, Oftung F, Korsvold GE, Geluk A, Ottenhoff TH. An unbiased genome-wide Mycobacteriumtuberculosis gene expression approach to discover antigens targeted by human Tcells expressed during pulmonary infection. J Immunol. 2013 Feb15;190(4):1659-71. doi: 10.4049/jimmunol.1201593. Epub 2013 Jan 14. (Medline PMID: 23319735)

    Nalbandian A, Yan BS, Pichugin A, Bronson RT, Kramnik I. Lung carcinogenesisinduced by chronic tuberculosis infection: the experimental model and geneticcontrol. Oncogene. 2009 Apr 30;28(17):1928-38. doi: 10.1038/onc.2009.32. Epub2009 Mar 30. (Medline PMID: 19330024)

    He X, Berland R, Mekasha S, Christensen TG, Alroy J, Kramnik I, Ingalls RR.The sst1 resistance locus regulates evasion of type I interferon signaling byChlamydia pneumoniae as a disease tolerance mechanism. PLoS Pathog.2013;9(8):e1003569. doi: 10.1371/journal.ppat.1003569. Epub 2013 Aug 29. (Medline PMID: 24009502)

    Colony and Husbandry Information

    Special Considerations

    Higher than average loss of litters, maintained on breeders chow and vitamin-enriched diet.

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    For more information about this colony's health status contact mmrrc_health@med.unc.edu

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Backross, then brother-sister mating
    Breeding Scheme(s)
    brother-sister mating
    Generation
    N12 (C57BL/6J), F20+
    Overall Breeding Performance
    Good

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 6 to 8 months 6 to 8 months
    Bred to Homozygosity
    No
    Average litter size
    4-6
    Recommended wean age
    3 weeks
    Average Pups Weaned
    4-6

    Order Request Information

    Availability Level

    The availability level for this product has not been determined.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    - Products for this strain are Not Yet Available for Ordering
    - If you register interest in this strain, you will be notified when it becomes available for ordering.
    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.