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Strain Detail Sheet

Strain Name:
B6J.Cg-Tg(Uty)1Psb/Mmucd
Stock Number:
043563-UCD
Citation ID:
RRID:MMRRC_043563-UCD
Other Names:
Uty

Strain Information

Gene Details

Tg(Uty)1Psb
Name: transgene insertion 1, Paul S Burgoyne
Type: Allele
Species: Mus musculus (mouse)
Chromosome: Y
Alteration at locus: Transgenic (BAC)
Uty
Name: ubiquitously transcribed tetratricopeptide repeat containing, Y-linked
Synonyms: Hydb
Type: Gene
Species: Mouse
Chromosome: Y
Alteration at locus: Transgenic (BAC)
NCBI: 22290

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
BAC clone CITB-246A22 was the main source of Uty. No individual BAC contained the full gene sequence; Burgoyne and colleagues used recombineering to add the missing exons as spliced cDNA sequence (exons 7-27). Founder line 1 was estimated by PMID:21093264 as having 8 copies.

Genotype Determination
Genotyping Protocol

Strain Description

Phenotype
Co-donor, Dr. Art Arnold communicated that the transgene expresses the RNA for a second gene, Ddx3y. However, this truncated RNA is unlikely to be expressed as protein.
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Apoptosis
  • Chromosome Pairing
  • Female
  • Fertility/genetics
  • Gene Silencing
  • Germ Cells/cytology
  • Germ Cells/physiology
  • Male
  • Meiosis/genetics
  • Mice
  • X Chromosome/genetics
  • Y Chromosome/genetics
  • Amino Acid Sequence
  • Apoptosis/physiology
  • Chromosome Pairing/physiology
  • DNA Primers/genetics
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Fluorescent Antibody Technique
  • Histological Techniques
  • In Situ Nick-End Labeling
  • Meiosis/physiology
  • Metaphase/physiology
  • Mice, Transgenic
  • Molecular Sequence Data
  • Ovary/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Sex Chromosomes/genetics
  • Sex-Determining Region Y Protein/genetics
  • Spermatocytes/physiology
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Transgenes/genetics
Strain Development
The Y BAC transgenic lines were produced originally by Paul Burgoyne (Francis Crick Institute, formerly National Institute for Medical Research, London) and Michael Mitchell (Aix Marseille Université, France), as part of their studies of Y genes that are critical for spermatogenesis. Classical methods of gene targeting were unsuccessful for Y genes, and thus Burgoyne and Mitchell used deletion mutants to find small regions of the Y chromosome that contain genes critical for spermatogenesis. Then, they added back Y genes as BAC transgenes, one by one, to determine which Y genes rescued the deficit in Y chromosome deletion variants with defined spermatogenic failure (e.g., PMID:11528390 and 21530259]). The Eif2s3x line was produced by Monika Ward at the University of Hawaii (PMID:24263135), who made the transgenic insertion using a BAC construct produced by Michael Mitchell. The 7 BAC transgenic lines were sent to Art Arnold (UCLA) on an MF1 or mixed MF1 background beginning in 2012, who backcrossed them for 10 or more generations to C57BL/6J. The Eif2s3x line was also backcrossed to MF1 at UCLA.

BAC DNA was injected as closed circles into fertilized eggs from CBA/Ca × C57BL/10J or randomly bred MF1 matings at the National Institute for Medical Research (PMID:21530259). The transgenic founders were crossed by the Burgoyne Lab to MF1 random-bred albino females, using BAC-specific primers listed below. In many cases, the copy number of the transgene was assessed by Southern-blot analysis (e.g., PMID:21093264). In cases where no single BAC encoded the entire gene, recombineering was used to add the missing exons as spliced cDNA.

This line is part of a group of related strains: MMRRC:43562, 43563, 43564, 43565, 43566, 43567, 43568, and 43694.

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Strain Origin

Donor
Paul S. Burgoyne, Ph.D., MRC National Institute for Medical Research.
Michael J. Mitchell, Ph.D., Inserm.
Arthur Arnold, Ph.D., University of California, Los Angeles.
Primary Reference

Royo H, Polikiewicz G, Mahadevaiah SK, Prosser H, Mitchell M, Bradley A, deRooij DG, Burgoyne PS, Turner JM. Evidence that meiotic sex chromosomeinactivation is essential for male fertility. Curr Biol. 2010 Dec7;20(23):2117-23. doi: 10.1016/j.cub.2010.11.010. Epub 2010 Nov 18. (Medline PMID: 21093264)

Vernet N, Mahadevaiah SK, Ojarikre OA, Longepied G, Prosser HM, Bradley A,Mitchell MJ, Burgoyne PS. The Y-encoded gene zfy2 acts to remove cells withunpaired chromosomes at the first meiotic metaphase in male mice. Curr Biol. 2011May 10;21(9):787-93. doi: 10.1016/j.cub.2011.03.057. Epub 2011 Apr 28. (Medline PMID: 21530259)

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Microbiome Analysis

Breeding

MMRRC Breeding System
Backcross
Generation
N10+ (C57BL/6J)

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Bred to Homozygosity
No

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
043563-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
043563-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
043563-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.