ENU-induced A to G transition at base pair 129,555,640 (v38) on chromosome 4, equivalent to base pair 18,002 in the GenBank genomic region NC_000070 encoding the Lck gene. The mutation corresponds to residue 953 in the NM_001162432 mRNA sequence in exon 9 of 13 total exons, or at position 995 bp of the NM_001162433 mRNA sequence in exon 9 of 13 total exons, or at position 1,050 of the NM_010693 mRNA sequence in exon 8 of 12 total exons. The mutation results in a glutamic acid to glycine substitution at position 299 (E299G) in the Lck protein isoform encoded by NM_001162432 and a glutamic acid to glycine substitution at position 288 (E288G) in the Lck protein isoforms encoded by NM_001162433 and NM_010693. The effect of the mutation on kinase activity, expression level, or localization has not been tested.

Homozygous: Homozygous swan mice lack T-dependent IgG responses to both aluminum hydroxide (alum)-emulsified ovalbumin (OVA) and recombinant Semliki Forest virus (rSFV)-β-galactosidase. Flow cytometric analysis of peripheral blood determined that swan mice exhibit a significant decrease in the frequency of CD4+ T cells and a slight decrease in the frequency of CD8+ T cells compared to the unaffected controls.

Heterozygous: Not determined

Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.

Wild-type littermates or C57BL/6J mice

The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact . Older strains may not have this information.