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Strain Name:
B6;129-Agrntm1Jrs/Mmucd
Stock Number:
015110-UCD
Citation ID:
RRID:MMRRC_015110-UCD
Other Names:
KOAGC, B6;129-Agrntm1Jrs/Mmcd

Strain Information

Agrntm1Jrs
Name: agrin; targeted mutation 1, Joshua R Sanes
Synonyms: agrnneo, z-agrin-, agrin-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Knockout
Agrn
Name: agrin
Synonyms: NMF380, Agrin, nmf380
Type: Gene
Species: Mouse
Chromosome: 4
Alteration at locus: Knockout
NCBI: 11603
HGNC: HGNC:329
Homologene: 27907
Genetic Alterations
A 743 bp region of the gene containing exons 32 and 33 was deleted and replaced with the neo cassette.
Genotype Determination

ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
Homozygous: Mutant animals are stillborn. Animals have severe presynaptic and postsynaptic neuromuscular defects. Mutant animals lack the agrin isoform required for acetylcholine receptor aggregation; other isoforms of agrin are produced, but are present at reduced levels.

Heterozygous: Same as wild-type.
Mammalian Phenotype Terms
Allelic Composition: Agrntm1Jrs/Agrntm1Jrs (Genetic Background: involves: 129S1/Sv * 129X1/SvJ )

Allelic Composition: Agrntm1Jrs/Agrntm1Jrs (Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 )

MeSH Terms
  • Agrin/genetics
  • Animals
  • Cell Differentiation/physiology
  • Cell Membrane/chemistry
  • Cell Membrane/physiology
  • Fetus/chemistry
  • Fetus/physiology
  • Gene Deletion
  • Gene Expression/physiology
  • Mice
  • Mice, Knockout
  • Mice, Mutant Strains
  • Muscle Fibers, Skeletal/cytology
  • Muscle Fibers, Skeletal/physiology
  • Muscle, Skeletal/embryology
  • Muscle, Skeletal/innervation
  • Muscle, Skeletal/physiology
  • Neuromuscular Junction/chemistry
  • Neuromuscular Junction/embryology
  • Presynaptic Terminals/chemistry
  • Presynaptic Terminals/physiology
  • Receptors, Cholinergic/physiology
  • Synapses/chemistry
  • Synapses/physiology
  • Transgenes/physiology
Strain Development
Chimeras were crossed with C57BL/6 animals to generate heterozygotes. Mutants are stillborn; the line has been maintained by crossing heterozygous siblings.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell Biology
  • Developmental Biology
  • Neurobiology
Donor
Joshua R Sanes, Ph.D. and Medha Gautam, Ph.D., St. Louis University Medical School
Primary Reference
Gautam M, Noakes PG, Moscoso L, Rupp F, Scheller RH, Merlie JP, Sanes JR. Defective neuromuscular synaptogenesis in agrin-deficient mutant mice. Cell. 1996 May 17;85(4):525-35. (Medline PMID: 8653788)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Agouti or black
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)

Wild-type female x Heterozygous male littermate or reciprocal mating

Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: N/A N/A
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 12 months 12 months
Average litter size
7-10
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
015110-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,568.61
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
015110-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $882.81
Non-Profit / For-Profit
Aliquot Approximate quantity3
015110-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.