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This model supports prolonged rhinovirus infection after further supplementation (via AAV-mediated delivery) with human stimulator of interferon genes (STING). This mouse may be of use to the virology community as a platform for further development of optimal rhinovirus mouse models.
A bacterial artificial chromosome (BAC) containing CDHR3 with point mutation C529Y (rs6967330, TGT->TAT), CTD-2262G10, was obtained from the human RPCI-11 BAC library. The appropriate modification was introduced into the BAC via bacterial recombination. This strategy generated a transgenic mouse line expressing the human CDHR3 gene carrying the C529Y variant under the control of the human CDHR3 promoter. CDHR3 exon 1 contained the translation initiation codon. The engineered CTD-2262G10 BAC was then used for pronuclear injection (PNI) into fertilized C57BL/6NTac oocytes. Human CDHR3 transgenic founder mice were made via PNI with random genome integration. After administration of hormones, superovulated C57BL/6NTac females were mated with C57BL/6NTac males. One cell stage embryos were isolated and microinjected with DNA solution. After recovery, 25–35 injected one cell-stage embryos were transferred to one of the oviducts of 0.5 dpc pseudopregnant NMRI females. Transgene integration was evaluated by using PCR. Founder mice were then bred with C57Bl/6NTac mice for one generation to achieve offspring heterozygous for hCDHR3 transgene expression. Mice were then interbred and mice homozygous for the transgene were identified by Taqman qPCR.
Goldstein ME, Ignacio MA, Loube JM, Whorton MR, Scull MA. Human Stimulator of Interferon Genes Promotes Rhinovirus C Replication in Mouse Cells In Vitro and In Vivo. Viruses. 2024 Aug 10;16(8):1282. doi: 10.3390/v16081282. (Medline PMID: 39205256)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.
The donor or their institution limits the distribution to non-profit institutions only.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
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