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Strain Name:
B6.SJL-dblr/Mmnc
Stock Number:
009994-UNC
Citation ID:
RRID:MMRRC_009994-UNC

Strain Information

dblr
Name: doubleridge
Synonyms: T3, Dkk1d
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Transgenic (random, gene disruption)
Genetic Alterations
A transgene concatamer inserted 150 kb downstream of Dkk1, causing deletion of approximately 60 kb of genomic DNA.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • Phenotype
    Homozygous: Postaxial polydactyly of the forelimbs; postaxial polydactyly of the right hind limb; microopthalmia or anopthalmia, anterior head defects, reduced survival.

    Hemizygous: Not characterized.
    Mammalian Phenotype Terms
    Allelic Composition: dblr/dblr (Genetic Background: involves: C57BL/6J * SJL/J )

    MeSH Terms
    • Alleles
    • Animals
    • Ectoderm/pathology
    • Extremities/embryology
    • Gene Expression Regulation, Developmental
    • Homeodomain Proteins/genetics
    • Homeodomain Proteins/metabolism
    • Intercellular Signaling Peptides and Proteins
    • Limb Deformities, Congenital/genetics
    • Mice
    • Mice, Mutant Strains
    • Proteins/genetics
    • Proteins/metabolism
    • Proto-Oncogene Proteins/genetics
    • Proto-Oncogene Proteins/metabolism
    • Wnt Proteins
    • Base Sequence
    • Chromosomes
    • Cloning, Molecular
    • DNA Transposable Elements
    • Exons
    • Fetal Death/genetics
    • Gene Dosage
    • Gene Rearrangement
    • Genetic Complementation Test
    • Head/abnormalities
    • Head/embryology
    • LDL-Receptor Related Proteins
    • Limb Deformities, Congenital/embryology
    • Low Density Lipoprotein Receptor-Related Protein-5
    • Low Density Lipoprotein Receptor-Related Protein-6
    • Mice, Transgenic
    • Molecular Sequence Data
    • Receptors, LDL/genetics
    • Receptors, LDL/metabolism
    • Signal Transduction
    • Spine/abnormalities
    • Spine/embryology
    • Syndactyly/genetics
    • Apoptosis/genetics
    • Body Patterning/genetics
    • Chromosome Mapping
    • Ectoderm/cytology
    • Fibroblast Growth Factor 8
    • Fibroblast Growth Factors/genetics
    • Forelimb/abnormalities
    • Forelimb/embryology
    • Hedgehog Proteins
    • In Situ Hybridization, Fluorescence
    • Male
    • Mice, Inbred C57BL
    • Mutation
    • Phenotype
    • Polydactyly/embryology
    • Polydactyly/genetics
    • Proto-Oncogene Proteins
    • Syndactyly/embryology
    • Trans-Activators/genetics
    Strain Development

    Array

    A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

    MMRRC Strain Reference Strains Genomic makeup (Mb)
    Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
    B6.SJL-dblr/Mmnc 9994 C57BL/6J SJL-J 2582 0 0 0
    Suggested Control Mice
    • Wildtype littermates
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Apoptosis
    • Cell Biology
    • Developmental Biology
    • Models for Human Disease
    Donor
    Miriam Meisler, Ph.D., University of Michigan
    Primary Reference
    • Adamska M, MacDonald BT, Sarmast ZH, Oliver ER, Meisler MH. En1 and Wnt7a interact with Dkk1 during limb development in the mouse. Dev Biol. 2004 Aug 1;272(1):134-44. (Medline PMID: 15242796)
    • MacDonald BT, Adamska M, Meisler MH. Hypomorphic expression of Dkk1 in the doubleridge mouse: dose dependence and compensatory interactions with Lrp6. Development. 2004 Jun;131(11):2543-52. Epub 2004 Apr 28. (Medline PMID: 15115753)
    • Adamska M, MacDonald BT, Meisler MH. Doubleridge, a mouse mutant with defective compaction of the apical ectodermal ridge and normal dorsal-ventral patterning of the limb. Dev Biol. 2003 Mar 15;255(2):350-62. (Medline PMID: 12648495)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    009994-UNC-RESUS Litter recovered from cryo-archive $3,088.00 / $5,126.00
    Non-Profit / For-Profit
    Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.