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Strain Detail Sheet

Strain Name:
B6(129S)-Et(EGFP/cre)16255Rdav/Mmmh
Stock Number:
034574-MU
Citation ID:
RRID:MMRRC_034574-MU
Major Collection:
Neuroscience Blueprint

Strain Information

Gene Details

Et(EGFP/cre)16255Rdav
Name: enhancer trap 16255, Ron Davis
Type: Allele
Species: Mus musculus (mouse)
Chromosome: UN
Alteration at locus: Transgenic
cre
Name: cre recombinase
Type: Gene
Species: Enterobacteria phage P1 (bacteriophage P1)
Chromosome:
Alteration at locus: Transgenic
EGFP
Name: enhanced green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
Alteration at locus: Transgenic
Genotype Determination

Strain Description

Phenotype
Homozygous: Homozygote phenotype is expected to be similar to the hemizygote phenotype.

Hemizygous: Mice hemizygous for this enhancer trap transgene are viable and fertile, with expression of an enhanced green fluorescent protein/Cre recombinase fusion protein (EGFP/Cre) gene under control of the promoter/enhancer regions surrounding the site where the enhancer trap transgene randomly inserted. Specifically, the donating investigator reports Cre recombinase activity in brain tissues as:
Cells in all areas; more cells in layers 4, 5, and 6 of the neocortex, hypothalamus, midbrain, pons, and medulla.
No Cre recombinase activity is reported in other tested tissues. The donating investigator did not characterize fluorescent expression of the EGFP portion of the fusion protein. When these enhancer trap lentiviral transgenic mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence(s) in cre-expressing tissues in the offspring.

The donating investigator reports that the transgene insertion site for line 16255 is located at 149.26 Mbp of chromosome 6. This is predicted to be within intron 1 of the RIKEN cDNA 2810474O19 gene locus (2810474O19Rik). No information was provided on whether or not the transgene insertion site disables the 2810474O19Rik locus.

Strain Development
An enhancer trap lentiviral transgenic approach was used to generate these mice. The Thy-EGFP-CRE transgene was designed with a self-inactivating lentiviral vector backbone (FUGW), a mouse Thy1.2 minimal promoter (-270 to +30), and an enhanced green fluorescent protein/Cre recombinase fusion protein (EGFP/Cre). This packaged lentivirus was injected subzonally (under the zona pellucida) into 1-2 cell stage C57BL/6J mouse embryos. Founder mice (F0) from line 16255 were bred to B6.R26-lacZ mutant mice (Gt(ROSA)26Sortm1Sor mutant mice backcrossed to C57BL/6 for at least 10 generations. Cre-positive transgenic pups (F1) were bred to C57BL/6 mice. The resulting pups (F2) were bred to C57BL/6 to obtain the F3 generation. The donating investigator reports that Cre-positive::lacZ-negative males from the F3 generation were used to freeze sperm, and that cryopreserved sperm were sent to the MMRRC. Upon arrival, aliquots of this frozen sperm were combined with oocytes from C57BL/6J inbred females to establish the live colony.
Suggested Control Mice

Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Research Tools

Strain Origin

Donor
Ronald L. Davis, Ph.D., The Scripps Research Institute.
Paul Overbeek, Ph.D., Baylor College of Medicine.
Primary Reference
Additional information regarding this strain can be found at NIH Neuroscience Blueprint Cre Driver Network.

Colony and Husbandry Information

Special Considerations

When maintaining a live colony, transgenic carrier mice may be bred together, to wild-type (non-carrier) siblings, or to C57BL/6J inbred mice.

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
034574-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
034574-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.