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Strain Name:
STOCK Gata4tm2(Gata4)Wtp/Mmjax
Stock Number:
032883-JAX
Citation ID:
RRID:MMRRC_032883-JAX

Strain Information

Gata4tm2(Gata4)Wtp
Name: GATA binding protein 4; targeted mutation 2, William T Pu
Synonyms: Gata4flap
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Knock-In
Gata4
Name: GATA binding protein 4
Synonyms: Gata-4
Type: Gene
Species: Mouse
Chromosome: 14
Alteration at locus: Knock-In
NCBI: 14463
HGNC: HGNC:4173
Homologene: 1551
Genetic Alterations
A floxed GATA4 cDNA with transcriptional stop cassette followed by a promoterless alkaline phosphatase cDNA was knocked into the 5' UTR of GATA4.
ES Cell Line
TC1/TC-1 derived from 129S6/SvEvTac
Phenotype
Homozygous: Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Cre-mediated excision of Gata4 is coupled with alkaline phosphatase expression under the control of the endogenous Gata4 regulatory elements. This mutant mouse strain may be useful in studies of cell lineage tracing in the developing and adult heart.

Heterozygous: Normal
MeSH Terms
  • Animals
  • Cell Differentiation/genetics
  • Cell Lineage/genetics
  • Cell Lineage/physiology
  • Embryo, Mammalian
  • Embryonic Stem Cells/metabolism
  • GATA4 Transcription Factor/genetics
  • GATA4 Transcription Factor/metabolism
  • Gene Expression Regulation, Developmental
  • Genes, Reporter
  • Heart
  • Homeobox Protein Nkx-2.5
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/physiology
  • Integrases/metabolism
  • LIM-Homeodomain Proteins
  • Mice
  • Mice, Inbred Strains
  • Mutation
  • Myocardium/cytology
  • Myocardium/metabolism
  • Myocytes, Cardiac/metabolism
  • Myocytes, Smooth Muscle/metabolism
  • Transcription Factors/genetics
  • Transcription Factors/physiology
Strain Development
A targeting vector containing a loxP-flanked Gata4 cDNA with a transcriptional stop cassette followed by an alkaline phosphatase cDNA and an FRT-flanked PGKneo cassette was inserted into the 5' UTR of the targeted gene. The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Chimeric animals were crossed to C57BL/6 and SJL mixed background Tg(ACTFLPe)9205Dym mice to remove the neomycin cassette. To remove the FLP transgene, mice were mated with outbred Black Swiss mice and then intercrossed to establish a homozygous colony. Upon arrival, mice were bred toC57BL/6J for at least 1 generation to establish the colony.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Cardiovascular
  • Developmental Biology
  • Models for Human Disease
  • Reproduction
  • Research Tools
Donor
William T. Pu, M.D., Boston Children's Hospital 
Primary Reference
Ma Q; Zhou B; Pu WT, Reassessment of Isl1 and Nkx2-5 cardiac fate maps using a Gata4-based reporter of Cre activity., Dev Biol 2008 Nov 1;323(1):98-104. (Medline PMID: 18775691)

Colony and Husbandry Information

While maintaining a live colony, these mice are bred as homozygotes.

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
032883-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
032883-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.