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Strain Detail Sheet

Strain Name:
B6N.129S5(B6)-Vsirtm1Lex/Mmucd
Stock Number:
031656-UCD
Citation ID:
RRID:MMRRC_031656-UCD
Other Names:
4632428N05Rik, UNQ730
Major Collection:
Genentech

Strain Information

Gene Details

Vsirtm1Lex
Name: V-set immunoregulatory receptor; targeted mutation 1, Lexicon Pharmaceuticals
Synonyms: PD-1H KO
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Knockout
Vsir
Name: V-set immunoregulatory receptor
Synonyms: VISTA, Dies1, PD-1H, 4632428N05Rik
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Knockout
NCBI: 74048
Homologene: 81923

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
The RIKEN cDNA 4632428N05 gene consists of 7 exons. Coding exon 1 was targeted (NCBI accession NM_028732.2) by homologous recombination.
Genotype Determination
Genotyping Protocol
ES Cell Line
Lexicon cell line derived from 129S5/SvEvBrd

Strain Description

Phenotype
Phenotypic analysis of the F2 mice indicates this mutation affects immunology and metabolism.
The phenotype of this strain may be different due to genetic background effects.

Phenotypic data provided by Lexicon Genetics, Inc.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
MeSH Terms
  • Animals
  • Membrane Proteins/genetics
  • Mice
  • Mice, Knockout
Strain Development
Targeted or gene trap mutations are generated in strain 129/SvEvBrd-derived embryonic stem (ES) cells. The chimeric mice are bred to C57BL/6-Tyrc-Brd albino mice albino mice to generate F1 heterozygous animals. These progeny are intercrossed to generate F2 wild type, heterozygous, and homozygous mutant progeny. On rare occasions, for example when very few F1 mice are obtained from the chimera, F1 heterozygous mice are crossed to 129/SvEvBrd x C57BL/6 hybrid mice to yield additional heterozygous animals for the intercross to generate the F2 mice.

Mice are archived at the backcross generation N12. Recovery will be performed using C57BL/6NCrl donors unless otherwise requested.
Suggested Control Mice
Wild-type littermates or C57BL/6NCrl mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Cell biology
  • Stem cell biology

Strain Origin

Donor
Genentech, Inc.
Primary Reference
Tang T, Li L, Tang J, Li Y, Lin W, Martin F, Deanna Grant D, Solloway M, Parker L, Weilan Ye W, Forrest W, Ghilardi N, Oravecz T, Platt KA, Rice DS, Hansen GM, Abuin A, Eberhart DE, Godowski P, Holt KH, Peterson A, Zambrowicz BP, de Sauvage FJ. A mouse knockout library for secreted and transmembrane proteins. Nat Biotechnol. 2010 July; 28(7):749–755. (Medline PMID: 20562862)

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)
  • Wild-type female x Heterozygous male
  • Heterozygous female x Heterozygous male
  • Homozygous female x Homozygous male
Generation
N12
Overall Breeding Performance
Unknown

Reproductive Statistics

Average litter size
Unspecified
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

Distribution of this strain requires execution of the Genentech Lexicon agreement. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
031656-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
031656-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
031656-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.