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Strain Name:
B6.129-Vegfatm2Gne/Mmucd
Stock Number:
071821-UCD
Citation ID:
RRID:MMRRC_071821-UCD
Other Names:
VEGF.flox.cko.B6N

Strain Information

Vegfatm2Gne
Name: vascular endothelial growth factor A; targeted mutation 2, Genentech
Synonyms: VEGFlox, Vegf-A/loxP, VEGFFlox, VEGFloxP(-), VEGF+f, VEGFloxP
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Conditional
Vegfa
Name: vascular endothelial growth factor A
Synonyms: VPF, VEGF-A, VEGF120, Vegf, VEGF188, VEGF164
Type: Gene
Species: Mouse
Chromosome: 17
Alteration at locus: Conditional
NCBI: 22339
Homologene: 2534
Genetic Alterations
Insertion of a loxP-flanked neomycin cassette and a loxP site flanking exon 3.
ES Cell Line
GS1 derived from 129/Sv
Phenotype
None/Normal/Wild-type
This mutation had no effect on the secretion of the protein.
MeSH Terms
  • Age Factors
  • Animals
  • Animals, Newborn/growth & development
  • Apoptosis
  • Body Constitution/physiology
  • Capillaries/cytology
  • Cell Division
  • Endothelial Growth Factors/genetics
  • Endothelium, Vascular/drug effects
  • Gene Targeting
  • Genes, Essential
  • Heart Defects, Congenital
  • Immunoglobulin G/genetics
  • Immunoglobulin G/pharmacology
  • Interferon-alpha/pharmacology
  • Kidney/abnormalities
  • Kidney/blood supply
  • Liver/abnormalities
  • Liver/blood supply
  • Lymphokines/genetics
  • Mice
  • Mice, Mutant Strains
  • Mutagenesis
  • Neovascularization, Physiologic
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins/pharmacology
  • Receptor Protein-Tyrosine Kinases/genetics
  • Receptor Protein-Tyrosine Kinases/pharmacology
  • Receptors, Growth Factor/genetics
  • Receptors, Vascular Endothelial Growth Factor
  • Recombinant Fusion Proteins/pharmacology
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factor Receptor-1
  • Vascular Endothelial Growth Factors
Strain Development
A VEGF genomic clone encompassing exons 1 to 4 of the murine VEGF locus was used to generate a BamHI-XbaI fragment encoding exon 2 of VEGF, which was blunt end cloned into the NotI site of TNLOX1-3 targeting vector. A XbaI-EcoRI fragment, encoding exon 3, was blunt end cloned into the AscI site and a EcoRI-KpnI fragment, including exon 4, was blunt end cloned into the PmeI site. All loxP-sites of the targeting vector TNBCE were sequenced and the orientation of the inserts verified by restriction digestion and sequencing.

The targeting vector was linearized by SalI digestion, then used for electroporation of the GS1 clone derived from 129Sv ES cells. Colonies were analyzed for complete recombination of the 3′ region, including the loxP-3 site, by PCR analysis. One derivative of each ES cell line containing the floxed VEGF allele was injected into the blastocoele cavity of 3.5-day C57BL/6J blastocysts. Chimeric males were mated with C57BL/6J females and agouti offsprings were screened for germline transmission by PCR analysis for VEGF alleles containing the loxP-1 and loxP-3 sites. The line was backcrossed to C57BL/6N for 13 generations to reach a congenic state.
Suggested Control Mice
wild-type
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cancer
  • Cardiovascular
Donor
Weilan Ye, Ph.D., Genentech, Inc.
Napoleone Ferrara, M.D., Genentech.
Primary Reference

Gerber HP, Hillan KJ, Ryan AM, Kowalski J, Keller GA, Rangell L, Wright BD, Radtke F, Aguet M, Ferrara N. VEGF is required for growth and survival in neonatal mice. Development. 1999 Mar;126(6):1149-59. doi: 10.1242/dev.126.6.1149. (Medline PMID: 10021335)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@ucdavis.edu
Coat Color
Black
Eye
Black
MMRRC Breeding System
Random intra-strain mating
Generation
13
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at mmrrc@ucdavis.edu for more details.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
071821-UCD-HET-F
071821-UCD-HET-M
Heterozygous / Hemizygous Female
Heterozygous / Hemizygous Male
$272.50 / Non-Profit Per Mouse The mmrrc@ucdavis.edu may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.