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Strain Detail Sheet

Strain Name:
B6(Cg)-Lgr5tm2(Hbegf/EGFP)Fjs/Mmucd
Stock Number:
071807-UCD
Citation ID:
RRID:MMRRC_071807-UCD
Other Names:
Lgr5-DTR/EGFP

Strain Information

Gene Details

Lgr5tm2(Hbegf/EGFP)Fjs
Name: leucine rich repeat containing G protein coupled receptor 5; targeted mutation 2, Frederick J de Sauvage
Synonyms: Lgr5DTR-EGFP, Lgr5DTR, Lgr5-EGFP-DTR, Lgr5tm2(DTR/EGFP)Fjs, Lgr5-DTR-GFP
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Knock-In
Lgr5
Name: leucine rich repeat containing G protein coupled receptor 5
Synonyms: Gpr49
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Knock-In
NCBI: 14160
HGNC: HGNC:4504
Homologene: 20807

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
A targeting vector was generated so that a DTR-EGFP-pA-loxP-neo-loxP cassette (diphtheria toxin (DT) cDNA linked in-frame to EGFP polyA-floxed neo) was inserted immediately after the ATG codon in exon 1 of the Lgr5 locus (deleting the remainder of exon 1 and splice donor site in intron 1 - a 212 bp deletion) by homologous recombination in C2 ES cells. Correctly targeted clones were transfected with a cre plasmid to remove the neo cassette.
ES Cell Line
C2 derived from C57BL/6NTac

Strain Description

Phenotype
Heterozygous mice are fertile and healthy, with no observed phenotypes. The model allows labeling and/or ablation of Lgr5+ (GPR49+Gpr49+) intestinal stem cells. The allele is homozygous lethal due to lack of LGR5 protein (Lgr5 KO phenotype).
MeSH Terms
  • Animals
  • Cell Lineage
  • Epithelial Cells/cytology
  • Epithelial Cells/metabolism
  • Female
  • Heparin-binding EGF-like Growth Factor
  • Homeostasis
  • Humans
  • Intercellular Signaling Peptides and Proteins/genetics
  • Intercellular Signaling Peptides and Proteins/metabolism
  • Intestine, Small/cytology
  • Intestine, Small/metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Nuclear Proteins/metabolism
  • Polycomb Repressive Complex 1
  • Proto-Oncogene Proteins/metabolism
  • Receptors, G-Protein-Coupled/deficiency
  • Receptors, G-Protein-Coupled/genetics
  • Receptors, G-Protein-Coupled/metabolism
  • Regeneration
  • Repressor Proteins/metabolism
  • Stem Cells/cytology
  • Stem Cells/metabolism
Strain Development
A 7,213 bp fragment from a C57BL/6 BAC (RP23 library) was first retrieved into plasmid pBlight-TK25. To generate the DTR–EGFP KI vector for Lgr5, a DTR–EGFP-pA-loxP-Neo-loxP cassette was synthesized and inserted right after the ATG of Lgr5, deleting the remainder of exon 1 and splice donor of intron 1 (a 212 bp deletion). To generate the CreERT2 KI vector, a dsRed2-IRES-CreERT2-pA-Frt-neo-Frt cassette was synthesized and inserted at the same position as the DTR–EGFP cassette. The Lgr5 KI vectors were linearized with NotI. C57BL/6 C2 embryonic stem cells were targeted using standard methods. Positive clones were identified using PCR and taqman analysis, and confirmed by sequencing of the modified locus. Correctly targeted embryonic stem cells were transfected with a Cre to remove the Neo cassette. The modified embryonic stem cells were then injected into blastocysts and germline transmission was obtained after crossing the resulting chimaeras with C57BL/6N females. The colony has been maintained by intercrossing and backcrossing (F23 and N15) to B6 albino (Strain #:000058).
Suggested Control Mice
Wild-type

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Cancer
  • Immunology and Inflammation
  • Research Tools

Strain Origin

Donor
Frederic De Sauvage, Ph.D., Genentech.
Primary Reference

Tian H, Biehs B, Warming S, Leong KG, Rangell L, Klein OD, de Sauvage FJ. A reserve stem cell population in small intestine renders Lgr5-positive cells dispensable. Nature. 2011 Sep 18;478(7368):255-9. doi: 10.1038/nature10408. Erratum in: Nature. 2012 Feb 2;482(7383):120. (Medline PMID: 21927002)

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat Color
White
Eye
Pink

Breeding

MMRRC Breeding System
Random intra-strain mating
Generation
15
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: N/A N/A
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
071807-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
071807-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
071807-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.