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Strain Detail Sheet

Strain Name:
C57BL/6J-Nfe2l2em1Mmaj/Mmnc
Stock Number:
071776-UNC
Citation ID:
RRID:MMRRC_071776-UNC
Other Names:
LSL-Nfe2l2E79Q

Strain Information

Gene Details

Nfe2l2em1Mmaj
Name: nuclear factor, erythroid derived 2, like 2; endonuclease-mediated mutation 1, Michael B Major
Synonyms: Nfe2l2E79Q
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: CRISPR
Nfe2l2
Name: nuclear factor, erythroid derived 2, like 2
Synonyms: Nrf2
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: CRISPR
NCBI: 18024
HGNC: HGNC:7782
Homologene: 2412

Additional Resources
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Google
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Gene Ontology
BioGPS
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
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GeneCards
ClinGen
Genetic Alterations
C57BL/6J mice were microinjected with CRISPR/Cas9 genome editing technology and a LOX-STOP-LOX (LSL) cassette. This cassette replaced the first intron 180 bp upstream exon 2 in the Nfe2l2 gene and included an E79Q mutation inside exon 2.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    Not applicable

    Strain Description

    Phenotype

    Nullizygous mice have increased sensitivity to oxidative stress in organs and cells, abnormal tooth enamel, altered response to injuries/chemical treatments, age-related decline in spermatogenesis and ovarian follicle numbers

    Conditional phenotype: expression of the mutant Nfe2l2-E79Q allele is silent until exposed to Cre recombinase. Activation of the Nfe2l2-E79Q allele in all K14-positive alleles driven by a K14-Cre allele. This resulted in hyperplasia/hyperkeratosis in the epithelium of the forestomach, esophagus and oral cavity. Other phenotypes observed included: stunted body axis, decreased weight, and decreased visceral adipose depots.

    MeSH Terms
    • Adipose Tissue, White/pathology
    • Animals
    • Carcinogenesis/genetics
    • Carcinoma, Squamous Cell/genetics
    • Disease Models, Animal
    • Esophagus/pathology
    • Humans
    • Hyperplasia/genetics
    • Mice
    • Mutation
    • NF-E2-Related Factor 2/genetics
    • Precancerous Conditions/genetics
    • Tongue/pathology
    • Upper Gastrointestinal Tract/pathology
    Strain Development
    A donor vector comprised a 2.3 kb segment of the mouse Nfe2l2 gene encompassing exons 2 and 3, including the E79Q mutation in exon 2, several silent mutations to remove PAM sites, and a LoxP-flanked stop cassette. This cassette was microinjected with CRISPR/Cas9 reagents into C57BL/6J embryos, where homologous recombination with the donor vector occurred in the endogenous Nfe2l2 gene.Pups were screened to identify founder animals harboring the correct knock-in allele. A male founder with the knock-in allele was mated to wild-type C57BL/6J females to produce F1 heterozygous pups.
    Suggested Control Mice
    C57BL/6J or untreated LSL-Nfe2l2E79Q mice.

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Cancer
    • Cell Biology
    • Immunology and Inflammation
    • Metabolism
    • Models for Human Disease

    Strain Origin

    Donor
    Bernard Weissman, Ph.D., University of North Carolina at Chapel Hill.
    Primary Reference

    Bowman BM, Montgomery SA, Schrank TP, Simon JM, Ptacek TS, Tamir TY, Mulvaney KM, Weir SJ, Nguyen TT, Murphy RM, Makowski L, Hayes DN, Chen XL, Randell SH, Weissman BE, Major MB. A conditional mouse expressing an activating mutation in NRF2 displays hyperplasia of the upper gastrointestinal tract and decreased white adipose tissue. J Pathol. 2020 Oct;252(2):125-137. doi: 10.1002/path.5504. Epub 2020 Aug 29. (Medline PMID: 32619021)

    Colony and Husbandry Information

    Special Considerations

    No

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Backcross
    Generation
    >20
    Overall Breeding Performance
    Excellent

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Greater than 12 months Greater than 12 months
    Average litter size
    7 to 9
    Recommended wean age
    4 Weeks
    Average Pups Weaned
    7 to 9

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin.

    Conditions of Distribution

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    071776-UNC-RESUS Litter recovered from cryo-archive $3,088.00 / $5,126.00
    Non-Profit / For-Profit    		 Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.