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Strain Detail Sheet

Strain Name:
B6J;129Sv-Thrb2tm3.2(cre)Df/Mmucd
Stock Number:
071594-UCD
Citation ID:
RRID:MMRRC_071594-UCD
Other Names:
Thrb2-Cre

Strain Information

Gene Details

Thrb
Name: thyroid hormone receptor beta
Synonyms: T3Rbeta, T3R[b], Nr1a2, TR beta, c-erbAbeta, Thrb2, Thrb1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 21834
Homologene: 36025

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Knock-In and Knock-Out uses a Cre cassette replacing the coding sequences in Thrb2 first exon, thus producing Cre enzyme instead of TRβ2 in Thrb2 active cells.
ES Cell Line
129Sv

Strain Description

Phenotype
Phenotype of Thrb2-Cre is same as Thrb2-ko.

In homologous Thrb2-Cre mice, retina photoreceptors lose M-opsin cones and compensate with the increase of S-opsin cones.

MeSH Terms
  • Animals
  • Mice
  • Gene Expression Regulation
  • Opsins/genetics
  • Receptors, Thyroid Hormone
  • Retina
  • Retinal Cone Photoreceptor Cells
  • Rod Opsins/genetics
Strain Development
Genomic Rorb 5′ and 3′ homology arms isolated from ES cell (derived from 129/Sv-+p+Tyr-cMgfSl-J/+ mice) DNA were inserted into a pACN vector carrying a self-excisable neomycin-resistance gene (tACE-Cre-Neo). A Cre cDNA replaced the Trb2-specific exon. Targeted W9.5 ES cell clones were determined by Southern blot and used to generate chimeric mice. Chimeras were crossed with C57BL/6J mice, the ACN cassette self-excised during germline transmission from male chimeras to yield the Trb2Cre allele. Tail-DNA PCR to confirm genotype. After backcrossing for 2 generations onto C57BL/6J, the Trb2+/Cre mice were crossed with adequate reporter strains for further analysis.
Suggested Control Mice
wild-type C57BL/6J

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Developmental Biology
  • Neurobiology
  • Sensorineural

Strain Origin

Donor
Douglas Forrest, Ph.D., NIDDK, NIH.
Primary Reference

Aramaki M, Wu X, Liu H, Liu Y, Cho YW, Song M, Fu Y, Ng L, Forrest D. Transcriptional control of cone photoreceptor diversity by a thyroid hormone receptor. Proc Natl Acad Sci U S A. 2022 Dec 6;119(49):e2209884119. doi: 10.1073/pnas.2209884119. Epub 2022 Dec 1. (Medline PMID: 36454759)

Colony and Husbandry Information

Special Considerations

No

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Sib-mating
Generation
N>2
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: N/A N/A
Age Reproductive Decline: Undetermined Undetermined
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
071594-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.