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Strain Detail Sheet

Strain Name:
C57BL/6J-Fgaem1Mjfk/Mmnc
Stock Number:
071592-UNC
Citation ID:
RRID:MMRRC_071592-UNC
Other Names:
Fga270

Strain Information

Gene Details

Fgaem1Mjfk
Name: fibrinogen alpha chain; endonuclease-mediated mutation 1, Matthew J Flick
Synonyms: Fga270 line A
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Endonuclease-mediated
Fga
Name: fibrinogen alpha chain
Synonyms: Fib, ENSMUSG00000059807
Type: Gene
Species: Mouse
Chromosome: 3
Alteration at locus: Endonuclease-mediated
NCBI: 14161
HGNC: HGNC:3661
Homologene: 428

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
CRISPR/Cas9 introduced a premature termination (stop) codon in exon 5 to generate a truncated mutation in the alpha chain region of fibrinogen. Resulting in a targeted ACGT to TTAA substitution.

Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    Not applicable

    Strain Description

    Phenotype
    Heterozygous: Normal
    Homozygous: Mild bleeding phenotype, dams unable to support pregnancy

    Some offspring had perinatal abdominal hemorrhagic events and soft-tissue bleeds within 3 days of birth; further, Adults didn’t have spontaneous events or altered survival rate
    MeSH Terms
    • Afibrinogenemia/genetics
    • Afibrinogenemia/metabolism
    • Animals
    • Blood Platelets/metabolism
    • Fibrinogen/genetics
    • Fibrinogen/metabolism
    • Hemostasis/genetics
    • Mice
    • Mice, Knockout
    • Mutation
    • Platelet Aggregation/genetics
    • Thrombosis/genetics
    • Thrombosis/metabolism
    Strain Development
    Single-cell C57Bl/6J embryos were injected with Cas9 protein, donor single-stranded DNA replacement vector and single guide RNA targeting the mouse Fga gene within exon 5. Injected embryos were subsequently implanted into pseudopregnant female mice to generate mice carrying the Fga270 mutation. Homologous recombinants were identified by polymerase chain reaction (PCR) analysis followed by a diagnostic restriction enzyme digest.
    Suggested Control Mice
    Wildtype C57Bl/6J

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Cancer
    • Cardiovascular
    • Diabetes
    • Hematology
    • Immunology and Inflammation
    • Metabolism
    • Models for Human Disease
    • Obesity
    • Research Tools

    Strain Origin

    Donor
    Matthew Flick, Ph.D., The University of North Carolina- Chapel Hill.
    Primary Reference

    Hur WS, Paul DS, Bouck EG, Negrón OA, Mwiza JM, Poole LG, Cline-Fedewa HM, Clark EG, Juang LJ, Leung J, Kastrup CJ, Ugarova TP, Wolberg AS, Luyendyk JP, Bergmeier W, Flick MJ. Hypofibrinogenemia with preserved hemostasis and protection from thrombosis in mice with an Fga truncation mutation. Blood. 2022 Mar 3;139(9):1374-1388. doi: 10.1182/blood.2021012537. (Medline PMID: 34905618)

    Colony and Husbandry Information

    Special Considerations

    Must mate female heterozygous mice due to bleeding tendency.

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Random intra-strain mating
    Generation
    N/A
    Overall Breeding Performance
    Excellent

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: No Yes
    Heterozygotes are fertile: N/A N/A
    Age Reproductive Decline: Greater than 12 months Greater than 12 months
    Average litter size
    7 to 9
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    4 to 6

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin.

    Conditions of Distribution

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    071592-UNC-RESUS Litter recovered from cryo-archive $3,088.00 / $5,126.00
    Non-Profit / For-Profit    		 Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.