Loading...

Strain Detail Sheet

Strain Name:
B6.Cg-Angptl4em1Bsjd/Mmmh
Stock Number:
069753-MU
Citation ID:
RRID:MMRRC_069753-MU
Other Names:
Angptl4

Strain Information

Gene Details

Angptl4em1Bsjd
Name: angiopoietin-like 4; endonuclease-mediated mutation 1, Brandon S J Davies
Synonyms: Angptl4fl
Type: Allele
Species: Mus musculus
Chromosome: 17
Alteration at locus: Conditional
Angptl4
Name: angiopoietin-like 4
Synonyms: FIAF, BK89, HFARP, NG27
Type: Gene
Species: Mouse
Chromosome: 17
Alteration at locus: Conditional
NCBI: 57875
Homologene: 10755

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations

Angptl4fl mutant mice contain a conditional knockout of the Angpl4 gene. Using a CRISPR-targeting approach, loxP sites were inserted flanking exons 2 and 3.

ES Cell Line
NA

Strain Description

Phenotype

Angptl4fl mice have a wildtype phenotype. When they are crossed with AdipoCre to generate an adipose-specific knockout, mice manifest lower plasma triglycerides and higher adipose lipoprotein lipase activity. When crossed with Albumin-Cre mice to generate a liver-specific knockout mice, plasma triglyceride levels are normal unless mice are fasted overnight or longer. With a longer fast mice manifest lower plasma triglyceride levels.

Strain Development
CRISPR guides, Cas9 protein, and a homology donor long single stranded DNA were microinjected into B6SJLF1/J zygotes and progeny were screened for the appropriate insertion of 5′ and 3′ LoxP sites by PCR amplification of the genomic region and subsequent sequencing. A male mouse containing both 5’ and 3’ LoxP sites was mated to C57BL/6J breeders (Jackson Labs Stock #000664) to produce male and female heterozygote mice, identified by PCR. Heterozygous progeny were interbred to generate homozygous floxed mice for initial phenotyping experiments. For cryopreservation and ongoing studies, heterozygote mice were backcrossed to C57BL/6J mice (Jackson Labs Stock #000664) for 10 generations. Then heterozygote mice were interbred to generate homozygous floxed mice.
Suggested Control Mice
For most experiments homozygous floxed mice without Cre are used as controls for homozygous flox Cre positive mice. To examine phenotypes of the conditional allele before Cre recombination, wild-type mice from a heterozygous flox mouse cross would be the control.

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Cardiovascular
  • Diabetes
  • Metabolism
  • Obesity

Strain Origin

Donor
Brandon Davies, Ph.D., University of Iowa.
Primary Reference

Spitler KM, Shetty SK, Cushing EM, Sylvers-Davie KL, Davies BSJ. Regulation of plasma triglyceride partitioning by adipose-derived ANGPTL4 in mice. Sci Rep. 2021 Apr 12;11(1):7873. doi: 10.1038/s41598-021-87020-5. (Medline PMCID: 8041937)

Spitler KM, Shetty SK, Cushing EM, Sylvers-Davie KL, Davies BSJ. Chronic high-fat feeding and prolonged fasting in liver-specific ANGPTL4 knockout mice. Am J Physiol Endocrinol Metab. 2021 Oct 1;321(4):E464-E478. doi: 10.1152/ajpendo.00144.2021. Epub 2021 Aug 16. (Medline PMCID: 8560380)

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Generation
10
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 4 to 6 months 7 to 9 months
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
069753-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
069753-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.