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Strain Detail Sheet

Strain Name:
129S6/SvEvTac-Slc12a1tm1Tkh/Mmnc
Stock Number:
000034-UNC
Citation ID:
RRID:MMRRC_000034-UNC

Strain Information

Gene Details

Slc12a1tm1Tkh
Name: solute carrier family 12, member 1; targeted mutation 1, Nobuyuki Takahashi
Synonyms: Slc12a1tm1Unc, NKCC2-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Knockout
Slc12a1
Name: solute carrier family 12, member 1
Synonyms: Nkcc2, mBSC1, D630042G03Rik, urehr3
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Knockout
NCBI: 20495
Homologene: 286

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
The promoter through part of intron 3 was deleted and replaced with a neo cassette.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    TC1/TC-1 derived from 129S6/SvEvTac

    Strain Description

    Phenotype
    Dehydration, inability to concentrate urine, death before or just after birth, none surviving past weaning without being treated.
    Mammalian Phenotype Terms
    Allelic Composition: Slc12a1tm1Tkh/Slc12a1tm1Tkh (Genetic Background: involves: 129S6/SvEvTac )
    MeSH Terms
    • Animals
    • Bartter Syndrome/genetics
    • Bartter Syndrome/pathology
    • Bartter Syndrome/physiopathology
    • Calcium/urine
    • Carrier Proteins/genetics
    • Carrier Proteins/physiology
    • Disease Models, Animal
    • Electrolytes/blood
    • Hydronephrosis/genetics
    • Hydronephrosis/physiopathology
    • Indomethacin/pharmacology
    • Introns
    • Kidney/metabolism
    • Kidney/pathology
    • Membrane Proteins/deficiency
    • Membrane Proteins/genetics
    • Membrane Proteins/physiology
    • Mice
    • Mice, Inbred Strains
    • Mice, Knockout
    • Polyuria/etiology
    • Polyuria/genetics
    • Polyuria/pathology
    • Potassium/blood
    • Promoter Regions, Genetic
    • Proteinuria
    • Renin/blood
    • Sodium-Potassium-Chloride Symporters
    Strain Development
    129SvEv coisogenic and B6.
    Suggested Control Mice
    • Wildtype littermates
    • 129S6/SvEvTac

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Water and electrolyte metabolism of the kidney

    Strain Origin

    Donor
    Drs. Oliver Smithies and Nobuyuki Takahashi,  University of North Carolina at Chapel Hill
    Primary Reference

    N.Takahashi,DR Chernavvsky,R.A. Gomez, P. Igarashi, H. Gitelman, O.Smithies, Uncompensated polyuria in a mouse model of Bartter's syndrome. Proc. Natl. Acad. Sci. USA 2000. 97:5434-39. (Medline PMID: 10779555)

    Colony and Husbandry Information

    Special Considerations

    Watch litters for small pups; remove pups that appear dehydrated -- they will die within 12 days of birth.

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Agouti

    Breeding

    MMRRC Breeding System
    Sib mating
    Breeding Scheme(s)
    • Heterozygous Female x Heterozygous Male
    Generation
    N6 As of: 11/2001
    Overall Breeding Performance
    Good

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: No No Homozygote pups die by 12 days of age.
    Homozygotes are fertile: No No
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Unknown Unknown
    Average litter size
    6
    Recommended wean age
    3 weeks

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    000034-UNC-SPERM Cryo-preserved spermatozoa $520.00 / Non-Profit Aliquot Approximate quantity3
    000034-UNC-RESUS Litter recovered from cryo-archive $3,088.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.